环状 VCAN/SUB1 通过上调 MYC/HSP90β 增强胶质瘤细胞的增殖和迁移。
CircVCAN/SUB1 up-regulates MYC/HSP90β to enhance the proliferation and migration of glioma cells.
机构信息
Department of Neurosurgery, Shanxi Bethune Hospital Shanxi Academy of Medical Sciences, No.99 Longcheng Street, Xiaodian District, Taiyuan, 030000, Shanxi, China.
Department of Respiratory, Gastroenterology and Oncology, the West Hospital of the Second Hospital of Shanxi Medical University, Taiyuan, 030053, Shanxi, China.
出版信息
Brain Res Bull. 2021 Dec;177:332-339. doi: 10.1016/j.brainresbull.2021.09.003. Epub 2021 Sep 14.
BACKGROUND
Gliomas are the most malignant and aggressive form of brain tumors, and account for the majority of brain cancer related deaths. Previous studies have reported that SUB1 serves as a novel RBP, which is highly expressed in tumors.
AIM
In our research, we aimed at exploring the regulatory mechanism of SUB1 in glioma cells.
METHODS
RT-qPCR was conducted to measure the expression of SUB1, HSP90β and MYC in glioma cells in U87 cell. Cell function tests were conducted to identify how the ectopic SUB1 expression influenced the proliferation and migration of glioma cells. Also, functional assays and mechanism experiments were carried out to figure out the relationship among circVCAN, SUB1 and MYC/HSP90β.
RESULTS
SUB1 expression was up-regulated in glioma cells. The inhibition of SUB1 expression suppressed the proliferation and migration of glioma cells, while the up-regulation of that accelerated the proliferation and migration of glioma cells. CircVCAN could up-regulate the expression of MYC/HSP90β by recruiting SUB1, thereby promoting glioma progression.
CONCLUSION
SUB1 plays a significant role in the survival and migration of glioma cells.
背景
神经胶质瘤是最恶性和侵袭性的脑肿瘤，占大多数与脑癌相关的死亡病例。先前的研究报告称，SUB1 作为一种新型的 RBP，在肿瘤中高度表达。
目的
在我们的研究中，我们旨在探索 SUB1 在神经胶质瘤细胞中的调控机制。
方法
使用 RT-qPCR 测量 U87 细胞中神经胶质瘤细胞中 SUB1、HSP90β 和 MYC 的表达。进行细胞功能试验以确定异位 SUB1 表达如何影响神经胶质瘤细胞的增殖和迁移。此外，进行功能测定和机制实验以阐明 circVCAN、SUB1 与 MYC/HSP90β 之间的关系。
结果
SUB1 在神经胶质瘤细胞中表达上调。抑制 SUB1 表达可抑制神经胶质瘤细胞的增殖和迁移，而上调 SUB1 则加速神经胶质瘤细胞的增殖和迁移。CircVCAN 可通过募集 SUB1 来上调 MYC/HSP90β 的表达，从而促进神经胶质瘤的进展。
结论
SUB1 在神经胶质瘤细胞的存活和迁移中发挥重要作用。
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