Department of Pharmacy, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, PR China.
Department of Pharmaceutical Analysis, School of Pharmacy, The Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou, 350122, China.
Anal Chim Acta. 2021 Sep 22;1179:338853. doi: 10.1016/j.aca.2021.338853. Epub 2021 Jul 15.
The content of deferasirox (DEF) in plasma is significant in β-thalassemia patient that needs long-term transfusion therapy, while the effective and simple strategy for DEF monitoring is still limited. The carbon dots (CDs) prepared from citric acid monohydrate and glutathione exhibit weakly modulated fluorescence intensity to several common metal ions containing Cu. Interestingly, the process of interaction of Cu and DEF forms the chelation of Cu and DEF (Cu-DEF) with the absorbance wavelength of DEF at 320 nm shifting to 332 nm for Cu-DEF. And the obtained Cu-DEF will effectively quench CDs through inner filter effect (IFE). Accordingly, a facile signal-off fluorescent method based on CDs as probe is developed for DEF detection using Cu as medium. And the proposed method exhibits linear range of 0.5-20 μg/mL with the detection limit of 0.33 μg/mL for DEF under the optimized conditions. Moreover, the developed assay is further expanded to test the content of DEF in dispersible tablet and plasma with accuracy and reproducibility. Such cost-effective and sensitive fluorescent assay just through simple mixing operation present a valuable strategy for drug monitoring.
去铁酮（DEF）在需要长期输血治疗的β-地中海贫血患者的血浆中的含量具有重要意义，而有效的 DEF 监测策略仍然有限。由一水柠檬酸和谷胱甘肽制备的碳点（CDs）对包含 Cu 的几种常见金属离子表现出弱的荧光强度调制。有趣的是，Cu 与 DEF 的相互作用过程形成了 Cu 和 DEF 的螯合物（Cu-DEF），DEF 的吸收波长从 320nm 转移到 332nm。并且获得的 Cu-DEF 将通过内滤效应（IFE）有效地猝灭 CDs。因此，开发了一种基于 CDs 作为探针的简单信号关闭荧光法，使用 Cu 作为介质来检测 DEF。在优化条件下，该方法对 DEF 的线性范围为 0.5-20μg/mL，检测限为 0.33μg/mL。此外，该方法进一步扩展到测定分散片和血浆中 DEF 的含量，具有准确性和重现性。这种具有成本效益且灵敏的荧光测定法仅通过简单的混合操作即可实现，为药物监测提供了一种有价值的策略。
