Latifi Fatemeh, Amjadi Mohammad, Hallaj Tooba, Alavi Seyyed Morteza Seyyed
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, 5166616471, Iran.
Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, Urmia University of Medical Sciences, Urmia, Iran.
Sci Rep. 2025 May 24;15(1):18075. doi: 10.1038/s41598-025-97035-x.
Tb doped carbon dots (Tb-CDs) were synthesized by a simple hydrothermal method and the existence of Tb in the obtained CDs was confirmed by the several characterization methods. Tb-CDs were exploited to design a ratiometric fluorescence sensor for deferasirox (DFX) assay. CDs and Tb acted as reference and sensing probs in the sensor. In the presence of DFX, the fluorescence intensity of CDs remained unchanged, while Tb emission increased. The fluorescence ratio of Tb to CDs was proportional to the DFX concentration at the range of 0.1 to 2.5 µM. Additionally, the fluorescence color altered from blue (CDs emission) to green (Tb emission) with increasing DFX concentration. We employed this color variation to establish a smartphone based sensor for the DFX detection. The linear range of established sensor was 0.5-15 µM. The detection limit (3S) for the ratiometric and smartphone based sensor was calculated to be 0.08 and 0.4 µM, respectively. Both sensors were applied to measure DFX in human serum samples with satisfactory results.
通过简单的水热法合成了掺铽碳点（Tb-CDs），并通过多种表征方法证实了所得碳点中铽的存在。利用Tb-CDs设计了一种用于测定地拉罗司（DFX）的比率荧光传感器。在该传感器中，碳点和铽分别作为参比探针和传感探针。在DFX存在的情况下，碳点的荧光强度保持不变，而铽的发射增强。在0.1至2.5 μM范围内，铽与碳点的荧光比率与DFX浓度成正比。此外，随着DFX浓度的增加，荧光颜色从蓝色（碳点发射）变为绿色（铽发射）。我们利用这种颜色变化建立了一种基于智能手机的DFX检测传感器。所建立传感器的线性范围为0.5 - 15 μM。基于比率法和智能手机的传感器的检测限（3S）分别计算为0.08和0.4 μM。两种传感器均应用于测定人血清样品中的DFX，结果令人满意。
