State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
J Biotechnol. 2022 Jan 10;343:32-37. doi: 10.1016/j.jbiotec.2021.09.009. Epub 2021 Sep 17.
Phenolic aldehydes are the major inhibitors from lignocellulose pretreatment. Previous studies show that oleaginous yeasts are difficult to survive in lignocellulosic hydrolysates even after the removal of furan aldehydes and organic acids inhibitors. This study investigated the cell viability, sugar consumption and lipid accumulation of the major oleaginous yeasts including Trichosporon cutaneum, Rhodosporidium toruloides, Rhodotorula glutinis, Yarrowia lipolytica in wheat straw hydrolysate containing only phenolic aldehydes after furan aldehydes and organic acids were selectively degraded by microorganisms. The results confirmed that the existence of residual phenolic aldehydes was the major reason for poor cell growth and metabolism of oleaginous yeasts. Only T. cutaneum demonstrated the higher tolerance by biodegrading phenolic aldehydes and the satisfactory cell growth and lipid production were obtained. This study revealed that T. cutaneum might be one of the promising cell factories for microbial lipid production from lignocellulosic feedstock.
酚醛类化合物是木质纤维素预处理的主要抑制剂。先前的研究表明，即使在去除糠醛和有机酸抑制剂后，油脂酵母也很难在木质纤维素水解物中存活。本研究在选择性降解糠醛和有机酸后，仅用酚醛类化合物处理小麦秸秆水解物，研究了主要油脂酵母（包括毛孢子菌、产朊假丝酵母、粘红酵母、解脂耶氏酵母）的细胞活力、糖消耗和脂积累情况。结果证实，残余酚醛类化合物的存在是油脂酵母生长和代谢不良的主要原因。只有毛孢子菌通过生物降解酚醛类化合物表现出更高的耐受性，从而获得了满意的细胞生长和油脂生产。本研究表明，毛孢子菌可能是利用木质纤维素原料生产微生物油脂的有前途的细胞工厂之一。
