Patelski Andrea Maria, Dziekońska-Kubczak Urszula, Nowak Agnieszka, Ditrych Maciej, Balcerek Maria, Pielech-Przybylska Katarzyna, Dziugan Piotr
Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, Poland.
Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, Poland.
Molecules. 2024 Nov 27;29(23):5607. doi: 10.3390/molecules29235607.
Numerous studies have demonstrated the efficacy of selenium compounds in preventing and treating lifestyle-related diseases such as cancer and cardiovascular disorders. The formulation of selenium-enriched supplements for humans and animals, particularly those containing selenium yeast, is highly advantageous. These products are rich in organic selenium derivatives, showing significantly higher bioavailability than inorganic forms of selenium. A particularly promising selenium analogue of sulphur-containing compounds is selenobiotin. The literature indicates that and strains can synthesise this compound. This research aimed to evaluate the effect of selenium supplementation on the biosynthesis of biotin and selenobiotin in and . The results have the potential to advance biotechnological approaches for the production of selenobiotin for various applications. A method based on affinity chromatography was used to quantify selenobiotin. The results confirmed that both yeast strains could synthesise selenobiotin in addition to biotin. In cells, selenobiotin accounted for up to 17.3% of the total biotin vitamer fraction. In comparison, in cells, it accounted for up to 28.4% of the sum of biotin and its analogues. The highest levels of selenobiotin were observed in cells cultured with selenomethionine.
大量研究已证明硒化合物在预防和治疗癌症及心血管疾病等与生活方式相关的疾病方面的功效。为人类和动物制备富含硒的补充剂，特别是那些含有硒酵母的补充剂，具有高度优势。这些产品富含有机硒衍生物，其生物利用度显著高于无机形式的硒。一种特别有前景的含硫化合物的硒类似物是硒生物素。文献表明，[具体菌株1]和[具体菌株2]菌株能够合成这种化合物。本研究旨在评估补充硒对[具体菌株1]和[具体菌株2]中生物素和硒生物素生物合成的影响。研究结果有可能推动用于各种应用的硒生物素生产的生物技术方法的发展。一种基于亲和色谱的方法被用于定量硒生物素。结果证实，两种酵母菌株除了能合成生物素外，还能合成硒生物素。在[具体菌株1]细胞中，硒生物素占生物素维生素原总组分的比例高达17.3%。相比之下，在[具体菌株2]细胞中，它占生物素及其类似物总和的比例高达28.4%。在用硒代蛋氨酸培养的细胞中观察到硒生物素的最高水平。
