Hu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing, 210095, China.
Hu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing, 210095, China.
Theriogenology. 2021 Nov;175:111-122. doi: 10.1016/j.theriogenology.2021.09.004. Epub 2021 Sep 11.
Inhibin subunit beta A (INHBA) participates in the synthesis of inhibin A, activin A and activin AB. Here we investigated the effect and molecular mechanism of INHBA on proliferation, apoptosis and hormone synthesis in sheep granulosa cells (GCs) using in vitro transfection. We first noticed that INHBA expression increased with follicle diameter and was widely distributed in ovarian tissue. The proliferation rate of GCs was significantly increased and decreased with overexpression and silence of INHBA, respectively, compared with the negative controls. INHBA transfection affected GC proliferation and apoptosis, regulating the expression of many cell cycle-related and apoptosis-related genes. INHBA overexpression significantly decreased activin and estradiol secretion while increasing inhibin and progesterone secretion. The expression of follicle-stimulating hormone beta subunit was significantly decreased and increased with INHBA overexpression and knockdown, respectively. Notably, silence of INHBA inhibited the expression of many transforming growth factor beta-related genes. Overall, the functional molecule of INHBA gene may be associated with follicular development via regulating proliferation, apoptosis and folliculogenesis-related hormone secretion of sheep GCs. In addition, our findings may contribute to a better understanding of the law of follicular development and thus improve the reproductive performance of female animals.
抑制素亚基β A（INHBA）参与抑制素 A、激活素 A 和激活素 AB 的合成。在这里，我们通过体外转染研究了 INHBA 对绵羊颗粒细胞（GC）增殖、凋亡和激素合成的影响及其分子机制。我们首先注意到，INHBA 表达随着卵泡直径的增加而增加，并广泛分布于卵巢组织中。与阴性对照相比，INHBA 的过表达和沉默分别显著增加和降低了 GC 的增殖率。INHBA 转染影响 GC 的增殖和凋亡，调节许多细胞周期相关和凋亡相关基因的表达。INHBA 过表达显著降低了激活素和雌二醇的分泌，而增加了抑制素和孕酮的分泌。卵泡刺激素β亚基的表达随着 INHBA 的过表达和敲低分别显著降低和增加。值得注意的是，INHBA 的沉默抑制了许多转化生长因子β相关基因的表达。总的来说，INHBA 基因的功能分子可能通过调节绵羊 GC 的增殖、凋亡和卵泡发生相关激素的分泌与卵泡发育有关。此外，我们的研究结果可能有助于更好地理解卵泡发育的规律，从而提高雌性动物的繁殖性能。
