• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

INHBA 基因沉默通过抑制 TGF-β 信号通路的激活抑制骨肉瘤细胞的增殖、迁移和侵袭。

INHBA gene silencing inhibits proliferation, migration, and invasion of osteosarcoma cells by repressing TGF-β signaling pathway activation.

机构信息

Second Department of Orthopaedics, The Third Affiliated Hospital of Qiqihar Medial University, Qiqihar, 161000, China.

Wuzhou Red Cross Hospital, Wuzhou, 543002, China.

出版信息

J Orthop Surg Res. 2023 Nov 8;18(1):848. doi: 10.1186/s13018-023-04330-2.

DOI:10.1186/s13018-023-04330-2
PMID:37940978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10634167/
Abstract

BACKGROUND

Osteosarcoma (OS) is a refractory malignancy. This study aimed to explore the roles and mechanisms of Inhibin subunit beta A (INHBA) in OS.

METHODS

INHBA expression levels in OS tissues and cells were assessed using RT-qPCR and western blotting. The impact of INHBA silencing on OS development was then explored by transfecting the OS cell lines U2OS and MG63 with INHBA-small interfering RNA (siRNA). The influence of INHBA silencing on U2OS and MG63 cell proliferation, migration, and invasion was examined using MTT and Transwell assays. Epithelial-mesenchymal transition (EMT) markers (E-cadherin and N-cadherin) were analyzed by RT-qPCR. The expression of genes involved in cell proliferation, migration, invasion, and the TGF-β signaling pathway was evaluated by western blotting and RT-qPCR.

RESULTS

INHBA levels were elevated in the OS tissues and cells. Furthermore, the transforming growth factor-β (TGF-β) signaling pathway of OS cells was suppressed in response to INHBA-siRNA, whereas proliferation, migration, and invasion of OS cells were inhibited. Besides, INHBA-siRNA significantly inhibited OS cell EMT, evidenced by enhanced E-cadherin mRNA expression and reduced N-cadherin mRNA expression. Further mechanistic studies revealed that the TGF-β1 agonist SRI-011381 hydrochloride increased OS cell proliferation, migration, and invasion after INHBA downregulation.

CONCLUSION

We found that INHBA silencing could play a vital role in OS via TGF-β1-regulated proliferation, migration, and invasion.

摘要

背景

骨肉瘤（OS）是一种难治性恶性肿瘤。本研究旨在探讨抑制素亚基β A（INHBA）在 OS 中的作用和机制。

方法

采用 RT-qPCR 和 Western blot 检测 OS 组织和细胞中 INHBA 的表达水平。然后，通过转染 OS 细胞系 U2OS 和 MG63 的 INHBA 小干扰 RNA（siRNA），探讨 INHBA 沉默对 OS 发展的影响。通过 MTT 和 Transwell 测定，研究 INHBA 沉默对 U2OS 和 MG63 细胞增殖、迁移和侵袭的影响。采用 RT-qPCR 分析上皮-间充质转化（EMT）标志物（E-钙粘蛋白和 N-钙粘蛋白）。通过 Western blot 和 RT-qPCR 评估与细胞增殖、迁移、侵袭和 TGF-β 信号通路相关的基因表达。

结果

INHBA 在 OS 组织和细胞中表达上调。此外，INHBA-siRNA 抑制 OS 细胞的转化生长因子-β（TGF-β）信号通路，同时抑制 OS 细胞的增殖、迁移和侵袭。此外，INHBA-siRNA 显著抑制 OS 细胞 EMT，表现为 E-钙粘蛋白 mRNA 表达增强和 N-钙粘蛋白 mRNA 表达降低。进一步的机制研究表明，TGF-β1 激动剂 SRI-011381 盐酸盐在 INHBA 下调后可增加 OS 细胞的增殖、迁移和侵袭。

结论

我们发现 INHBA 沉默可通过 TGF-β1 调节的增殖、迁移和侵袭在 OS 中发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/ce8dd2c4a925/13018_2023_4330_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/5bc74cdd54a9/13018_2023_4330_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/aa0b2623d256/13018_2023_4330_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/153f13d4357c/13018_2023_4330_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/bcccc8872cf7/13018_2023_4330_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/2a20e3b4c09f/13018_2023_4330_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/9fbad4412c86/13018_2023_4330_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/0c94409ee790/13018_2023_4330_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/4cf2d9cd9459/13018_2023_4330_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/3932f5523081/13018_2023_4330_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/ce8dd2c4a925/13018_2023_4330_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/5bc74cdd54a9/13018_2023_4330_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/aa0b2623d256/13018_2023_4330_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/153f13d4357c/13018_2023_4330_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/bcccc8872cf7/13018_2023_4330_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/2a20e3b4c09f/13018_2023_4330_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/9fbad4412c86/13018_2023_4330_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/0c94409ee790/13018_2023_4330_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/4cf2d9cd9459/13018_2023_4330_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/3932f5523081/13018_2023_4330_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8044/10634167/ce8dd2c4a925/13018_2023_4330_Fig10_HTML.jpg

相似文献

1
INHBA gene silencing inhibits proliferation, migration, and invasion of osteosarcoma cells by repressing TGF-β signaling pathway activation.INHBA 基因沉默通过抑制 TGF-β 信号通路的激活抑制骨肉瘤细胞的增殖、迁移和侵袭。
J Orthop Surg Res. 2023 Nov 8;18(1):848. doi: 10.1186/s13018-023-04330-2.
2
By targeting TRAF6, miR-140-3p inhibits TGF-β1-induced human osteosarcoma epithelial-to-mesenchymal transition, migration, and invasion.通过靶向 TRAF6，miR-140-3p 抑制 TGF-β1 诱导的人骨肉瘤上皮-间充质转化、迁移和侵袭。
Biotechnol Lett. 2020 Nov;42(11):2123-2133. doi: 10.1007/s10529-020-02943-9. Epub 2020 Jun 19.
3
INHBA gene silencing inhibits gastric cancer cell migration and invasion by impeding activation of the TGF-β signaling pathway.INHBA 基因沉默通过阻碍 TGF-β 信号通路的激活抑制胃癌细胞迁移和侵袭。
J Cell Physiol. 2019 Aug;234(10):18065-18074. doi: 10.1002/jcp.28439. Epub 2019 Apr 8.
4
Silencing of long-non-coding RNA ANCR suppresses the migration and invasion of osteosarcoma cells by activating the p38MAPK signalling pathway.长链非编码 RNA ANCR 的沉默通过激活 p38MAPK 信号通路抑制骨肉瘤细胞的迁移和侵袭。
BMC Cancer. 2019 Nov 14;19(1):1112. doi: 10.1186/s12885-019-6335-4.
5
Downregulation of inhibits proliferation and metastasis of osteosarcoma cells.下调 抑制骨肉瘤细胞的增殖和转移。
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2024 Jun 28;49(6):870-877. doi: 10.11817/j.issn.1672-7347.2024.240020.
6
Knockdown of CD44 inhibits proliferation, migration and invasion of osteosarcoma cells accompanied by downregulation of cathepsin S.敲低 CD44 抑制骨肉瘤细胞的增殖、迁移和侵袭，并伴随组织蛋白酶 S 的下调。
J Orthop Surg Res. 2022 Mar 9;17(1):154. doi: 10.1186/s13018-022-03048-x.
7
Glaucocalyxin A reverses EMT and TGF-β1-induced EMT by inhibiting TGF-β1/Smad2/3 signaling pathway in osteosarcoma.白杨素 A 通过抑制骨肉瘤中 TGF-β1/Smad2/3 信号通路逆转 EMT 和 TGF-β1 诱导的 EMT。
Chem Biol Interact. 2019 Jul 1;307:158-166. doi: 10.1016/j.cbi.2019.05.005. Epub 2019 May 4.
8
Integrin signaling potentiates transforming growth factor-beta 1 (TGF-β1) dependent down-regulation of E-Cadherin expression - Important implications for epithelial to mesenchymal transition (EMT) in renal cell carcinoma.整合素信号增强转化生长因子-β1（TGF-β1）依赖的E-钙黏蛋白表达下调——对肾细胞癌上皮-间质转化（EMT）的重要意义。
Exp Cell Res. 2017 Jun 15;355(2):57-66. doi: 10.1016/j.yexcr.2017.03.051. Epub 2017 Mar 29.
9
Chimaphilin inhibits human osteosarcoma cell invasion and metastasis through suppressing the TGF-β1-induced epithelial-to-mesenchymal transition markers via PI-3K/Akt, ERK1/2, and Smad signaling pathways.奇马菲林通过PI-3K/Akt、ERK1/2和Smad信号通路抑制TGF-β1诱导的上皮-间质转化标志物，从而抑制人骨肉瘤细胞的侵袭和转移。
Can J Physiol Pharmacol. 2018 Jan;96(1):1-7. doi: 10.1139/cjpp-2016-0522. Epub 2017 Jan 29.
10
Inhibin β-A (INHBA) induces epithelial-mesenchymal transition and accelerates the motility of breast cancer cells by activating the TGF-β signaling pathway.抑制素 β-A（INHBA）通过激活 TGF-β 信号通路诱导上皮-间充质转化并加速乳腺癌细胞的迁移。
Bioengineered. 2021 Dec;12(1):4681-4696. doi: 10.1080/21655979.2021.1957754.

引用本文的文献

1
Integrative single-cell transcriptomic analysis deciphers heterogeneous characteristics of gastrointestinal tract cancer.整合单细胞转录组分析揭示胃肠道癌的异质性特征。
Clin Transl Med. 2025 Aug;15(8):e70415. doi: 10.1002/ctm2.70415.
2
Biological effect of ETV4 and the underlying mechanism of its regulatory effect on epithelial‑mesenchymal transition in intrahepatic cholangiocarcinoma cells.ETV4对肝内胆管癌细胞的生物学效应及其调控上皮-间质转化的潜在机制
Oncol Lett. 2024 May 30;28(2):346. doi: 10.3892/ol.2024.14479. eCollection 2024 Aug.
3
INHBA is Enriched in HPV-negative Oropharyngeal Squamous Cell Carcinoma and Promotes Cancer Progression.

本文引用的文献

1
Upregulation of INHBA mediated by the transcription factor BHLHE40 promotes colon cancer cell proliferation and migration.转录因子 BHLHE40 介导的 INHBA 上调促进结肠癌细胞增殖和迁移。
J Clin Lab Anal. 2022 Jul;36(7):e24539. doi: 10.1002/jcla.24539. Epub 2022 Jun 11.
2
Comprehensive analysis of INHBA: A biomarker for anti-TGFβ treatment in head and neck cancer.全面分析 INHBA：头颈部癌症抗 TGFβ 治疗的生物标志物。
Exp Biol Med (Maywood). 2022 Aug;247(15):1317-1329. doi: 10.1177/15353702221085203. Epub 2022 May 6.
3
Tumor in the Crossfire: Inhibiting TGF-β to Enhance Cancer Immunotherapy.
INHBA 在 HPV 阴性口咽鳞状细胞癌中富集，并促进癌症进展。
Cancer Res Commun. 2024 Feb 28;4(2):571-587. doi: 10.1158/2767-9764.CRC-23-0258.
肿瘤在交火中：抑制 TGF-β 以增强癌症免疫治疗。
BioDrugs. 2022 Mar;36(2):153-180. doi: 10.1007/s40259-022-00521-1. Epub 2022 Mar 30.
4
CircTHBS1 drives gastric cancer progression by increasing INHBA mRNA expression and stability in a ceRNA- and RBP-dependent manner.环状 RNA THBS1 通过 ceRNA 和 RBP 依赖性方式增加 INHBA mRNA 表达和稳定性促进胃癌进展。
Cell Death Dis. 2022 Mar 25;13(3):266. doi: 10.1038/s41419-022-04720-0.
5
hsa-miR-875-5p inhibits tumorigenesis and suppresses TGF-β signalling by targeting USF2 in gastric cancer.hsa-miR-875-5p 通过靶向 USF2 抑制胃癌的肿瘤发生和 TGF-β 信号通路。
J Transl Med. 2022 Mar 7;20(1):115. doi: 10.1186/s12967-022-03253-6.
6
Inhibin subunit beta A promotes cell proliferation and metastasis of breast cancer through Wnt/β-catenin signaling pathway.抑制素亚基β A 通过 Wnt/β-连环蛋白信号通路促进乳腺癌细胞增殖和转移。
Bioengineered. 2021 Dec;12(2):11567-11575. doi: 10.1080/21655979.2021.1971028.
7
SPECT/CT in the Evaluation of Suspected Skeletal Pathology.SPECT/CT 评估疑似骨骼病变。
Tomography. 2021 Oct 11;7(4):581-605. doi: 10.3390/tomography7040050.
8
INHBA transfection regulates proliferation, apoptosis and hormone synthesis in sheep granulosa cells.INHBA 转染可调节绵羊颗粒细胞的增殖、凋亡和激素合成。
Theriogenology. 2021 Nov;175:111-122. doi: 10.1016/j.theriogenology.2021.09.004. Epub 2021 Sep 11.
9
INHBA is a novel mediator regulating cellular senescence and immune evasion in colorectal cancer.抑制素βA是一种调节结直肠癌细胞衰老和免疫逃逸的新型介质。
J Cancer. 2021 Aug 13;12(19):5938-5949. doi: 10.7150/jca.61556. eCollection 2021.
10
Inhibin β-A (INHBA) induces epithelial-mesenchymal transition and accelerates the motility of breast cancer cells by activating the TGF-β signaling pathway.抑制素 β-A（INHBA）通过激活 TGF-β 信号通路诱导上皮-间充质转化并加速乳腺癌细胞的迁移。
Bioengineered. 2021 Dec;12(1):4681-4696. doi: 10.1080/21655979.2021.1957754.