褪黑素在农场动物精子冷冻保存中的应用：简要综述。

Use of melatonin in sperm cryopreservation of farm animals: A brief review.

作者信息

Ofosu Jones, Qazi Izhar Hyder, Fang Yi, Zhou Guangbin

机构信息

College of Animal Science and Technology, and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

College of Animal Science and Technology, and Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China; Department of Veterinary Anatomy and Histology, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sakrand 67210, Sindh, Pakistan.

出版信息

Anim Reprod Sci. 2021 Sep 11;233:106850. doi: 10.1016/j.anireprosci.2021.106850.

DOI:10.1016/j.anireprosci.2021.106850

PMID:34537566

Abstract

Melatonin (MT) is a potent antioxidant with useful applications in several fields. Due to the capacity to scavenge free radicals and enhance cellular endogenous antioxidant defenses, MT is widely used in sperm cryopreservation to protect against oxidative stress-induced damage in frozen-thawed sperm. In this article, there is a review of positive effects of MT supplementation in cryopreservation of sperm from domestic ruminants and swine. There is direct or indirect scavenging of free radicals, preventing lipid peroxidation (LPO), and reducing oxidative stress, therefore, protecting membrane and DNA integrity, enhancing post-thaw antioxidant and enzymatic functions to maintain mitochondrial functions and activity, and regulating ATP production and utilization leading to maintenance of sperm quality, motility, and viability. In addition, MT reportedly inhibits sperm apoptosis, potentially by enhancing sperm viability and modulating abundances of mRNA transcripts.

摘要

褪黑素（MT）是一种强效抗氧化剂，在多个领域都有实用价值。由于其具有清除自由基和增强细胞内源性抗氧化防御的能力，MT被广泛应用于精子冷冻保存，以防止冻融精子受到氧化应激诱导的损伤。本文综述了补充MT对家畜反刍动物和猪精子冷冻保存的积极作用。MT可直接或间接清除自由基，防止脂质过氧化（LPO），减轻氧化应激，从而保护细胞膜和DNA的完整性，增强解冻后精子的抗氧化和酶功能，以维持线粒体功能和活性，调节ATP的产生和利用，进而维持精子质量、活力和生存能力。此外，据报道MT可抑制精子凋亡，可能是通过提高精子活力和调节mRNA转录本丰度来实现的。