Wang Xiaoyu, Shi Naiqi, Hui Meiqi, Jin Hui, Gao Shumei, Zhou Qiao, Zhang Li, Yan Meijuan, Shen Hongmei
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Department of Aoyang Cancer Institute, Affiliated Aoyang Hospital of Jiangsu University, Suzhou, China.
Front Cell Neurosci. 2021 Sep 3;15:723308. doi: 10.3389/fncel.2021.723308. eCollection 2021.
β-1,4 Galactosyltransferase V (β-1,4-GalT V) belongs to the β-1,4 galactosyltransferase family, which modifies proteins and plays a vital role in biological function. Our previous study revealed that β-1,4-GalT V was expressed in the cortex and hippocampus and participated in the recovery of spatial learning and memory in rats with traumatic brain injury. However, the expression of β-1,4-GalT V in microglia, resident immune cells in the central nervous system, and its impact on microglia in resting and lipopolysaccharide-triggered activated stages are elusive. In this study, we clarified that β-1,4-GalT V expresses in microglia, and it regulates microglial migration, proliferation, and release of the inflammatory factors. We also observed that β-1,4-GalT V affects the expression level of tumor necrosis factor receptor (TNFR)2 instead of TNFR1. These results strongly support the fact that β-1,4-GalT V is involved in microglial function.
β-1,4半乳糖基转移酶V（β-1,4-GalT V）属于β-1,4半乳糖基转移酶家族，该家族可修饰蛋白质并在生物学功能中发挥至关重要的作用。我们之前的研究表明，β-1,4-GalT V在大鼠的大脑皮质和海马体中表达，并参与创伤性脑损伤大鼠空间学习和记忆的恢复。然而，β-1,4-GalT V在小胶质细胞（中枢神经系统中的常驻免疫细胞）中的表达及其在静息和脂多糖触发的激活阶段对小胶质细胞的影响尚不清楚。在本研究中，我们阐明了β-1,4-GalT V在小胶质细胞中表达，并且它调节小胶质细胞的迁移、增殖以及炎性因子的释放。我们还观察到β-1,4-GalT V影响肿瘤坏死因子受体（TNFR）2而非TNFR1的表达水平。这些结果有力地支持了β-1,4-GalT V参与小胶质细胞功能这一事实。
