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基于代谢组学和转录组学分析对核桃脂质代谢的洞察

Insights Into Walnut Lipid Metabolism From Metabolome and Transcriptome Analysis.

作者信息

Yan Suxian, Wang Xingsu, Yang Chenkang, Wang Junyou, Wang Ying, Wu Bangbang, Qiao Ling, Zhao Jiajia, Mohammad Pourkheirandish, Zheng Xingwei, Xu Jianguo, Zhi Huming, Zheng Jun

机构信息

State Key Laboratory of Sustainable Dryland Agriculture, Institute of Wheat Research, Shanxi Agricultural University, Linfen, China.

College of Food Science, Shanxi Normal University, Linfen, China.

出版信息

Front Genet. 2021 Sep 3;12:715731. doi: 10.3389/fgene.2021.715731. eCollection 2021.

DOI:10.3389/fgene.2021.715731
PMID:34539744
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8446449/
Abstract

Walnut oil is an excellent source of essential fatty acids. Systematic evaluation of walnut lipids has significance for the development of the nutritional and functional value of walnut. Ultra-performance liquid chromatography/Orbitrap high-resolution mass spectrometry (UHPLC-Orbitrap HRMS) was used to characterize the lipids of walnut. A total of 525 lipids were detected and triacylglycerols (TG) (18:2/18:2/18:3) and diacylglycerols (DG) (18:2/18:2) were the main glycerolipids present. Essential fatty acids, such as linoleic acid and linolenic acid, were the main DG and TG fatty acid chains. Many types of phospholipids were observed with phosphatidic acid being present in the highest concentration (5.58%). Using a combination of metabolome and transcriptome analysis, the present study mapped the main lipid metabolism pathway in walnut. These results may provide a theoretical basis for further study and specific gene targets to enable the development of walnut with increased oil content and modified fatty acid composition.

摘要

核桃油是必需脂肪酸的优质来源。对核桃脂质进行系统评估对于核桃营养和功能价值的开发具有重要意义。采用超高效液相色谱/轨道阱高分辨率质谱（UHPLC-Orbitrap HRMS）对核桃脂质进行表征。共检测到525种脂质，其中三酰甘油（TG）（18:2/18:2/18:3）和二酰甘油（DG）（18:2/18:2）是主要的甘油脂质。必需脂肪酸，如亚油酸和亚麻酸，是主要的DG和TG脂肪酸链。观察到多种类型的磷脂，其中磷脂酸的浓度最高（5.58%）。本研究结合代谢组学和转录组学分析，绘制了核桃主要的脂质代谢途径。这些结果可为进一步研究和特定基因靶点提供理论依据，以培育含油量增加且脂肪酸组成改良的核桃。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/1ddf86c7e889/fgene-12-715731-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/b9c61b23619f/fgene-12-715731-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/95393d77aa2b/fgene-12-715731-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/4bfd32d87387/fgene-12-715731-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/a253e20c2741/fgene-12-715731-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/621b777e37fe/fgene-12-715731-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/209f79e9dc88/fgene-12-715731-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/1ddf86c7e889/fgene-12-715731-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/b9c61b23619f/fgene-12-715731-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/95393d77aa2b/fgene-12-715731-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/4bfd32d87387/fgene-12-715731-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/a253e20c2741/fgene-12-715731-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/621b777e37fe/fgene-12-715731-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/209f79e9dc88/fgene-12-715731-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8195/8446449/1ddf86c7e889/fgene-12-715731-g007.jpg

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