Rajeoni Ali Hojabr, Ghalyanchilangeroudi Arash, Khalesi Bahman, Madadi Mohammad Sadegh, Hosseini Hossein
Department of Microbiology and Immunology, School of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AEREO), Karaj, Iran.
Iran J Microbiol. 2021 Jun;13(3):337-344. doi: 10.18502/ijm.v13i3.6396.
Avian respiratory disease complex (RDC) is one of the most detrimental economic diseases that affected different parts of the world. Various pathogens cause the disease, but the most significant viral pathogens include avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) are the most prevalent. To detect these pathogens, various methods have been discovered in the last decades. Detection and characterization of viruses by metagenomics methods have improved our knowledge about the role of virome in the avian complex respiratory disease.
This research investigates the viral pathogen populations that mostly participate in emerging these diseases using the NGS method RNA-sequencing. In surveillance of ten broiler farms from different cities with respiratory symptoms, trachea samples were collected to determine the pathogenic virome causing the disease.
In this metagenomics analysis, nine viral families were identified, comprising 72.82% of RNA viruses, 24.32% of RT viruses, and 2.86% of DNA viruses. RNA viruses had the highest contribution to the respiratory disease complex instead of disease, including paramyxoviridae, orthomyxoviridae, coronaviridae, and picornaviridae viruses. Other viruses from the RNA viruses and DNA virus families were also identified in addition to these results.
This research suggests that studies of pathogenic viromes in different diseases can help monitor different diseases and predict their future occurrence.
禽呼吸道疾病综合征（RDC）是影响世界不同地区的最具危害性的经济疾病之一。多种病原体可引发该疾病，但最重要的病毒病原体包括禽流感病毒（AIV）、传染性支气管炎病毒（IBV）和新城疫病毒（NDV）最为常见。在过去几十年中，已发现多种检测这些病原体的方法。通过宏基因组学方法对病毒进行检测和鉴定，提高了我们对病毒组在禽复杂呼吸道疾病中作用的认识。
本研究采用NGS方法RNA测序，调查主要参与这些疾病发生的病毒病原体群体。在对来自不同城市有呼吸道症状的10个肉鸡场进行监测时，采集气管样本以确定引起疾病的致病病毒组。
在这项宏基因组学分析中，鉴定出9个病毒科，其中RNA病毒占72.82%，逆转录病毒占24.32%，DNA病毒占2.86%。RNA病毒对呼吸道疾病综合征的贡献最大，而非疾病本身，包括副粘病毒科、正粘病毒科、冠状病毒科和小RNA病毒科病毒。除这些结果外，还鉴定出了来自RNA病毒科和DNA病毒科的其他病毒。
本研究表明，对不同疾病中致病病毒组的研究有助于监测不同疾病并预测其未来发生情况。
