Asadi Sepideh, Soleimani Neda, Babadi Zahra Khosravi, Ebrahimipour Gholam Hossein
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Iran J Microbiol. 2021 Jun;13(3):372-380. doi: 10.18502/ijm.v13i3.6400.
Cancer incidence and recurrence, antibiotic resistance, and overuse of antibiotics have become a global concern. The purpose of this study was to identify and isolate bacteria from the skin of the , Iranian swamp frog, which has produced antimicrobial compounds, and investigate its cytotoxic activity on the breast (MCF7) and glioblastoma (U87) cancer cell line.
An antibiotic-producing bacterium was isolated from the frog skin. The bacterium was identified based on 16S rDNA sequencing and biochemical and morphological characteristics. Antimicrobial activity of the culture supernatant was examined by disc diffusion and MIC methods. Cytoplasmic and cell wall extracts of bacteria were prepared by sonication. SDS-PAGE was then used to examine protein contents of them. The cancer cell lines were treated with cytoplasmic and cell wall extracts at different concentrations. The effects of cytotoxicity were assessed by MTT assay at 24 and 48 h intervals. Finally, the results were analyzed by SPSS.
The isolated bacterium was identified as a new strain of . MIC and disc diffusion methods showed that the antimicrobial activity was broad spectrum. MTT assay showed IC50 values 30 μg/ml and 20 μg/ml for U87 and MCF7 cells after 24-48 h exposure, respectively.
The cytoplasmic extracts of has anticancer potential and can be used as an alternative or complementary candidate in the treatment of cancer. Further and mechanistic studies are suggested to confirm the biological activities.
癌症发病率与复发、抗生素耐药性以及抗生素的过度使用已成为全球关注的问题。本研究的目的是从伊朗沼泽蛙的皮肤中鉴定并分离出产生抗菌化合物的细菌，并研究其对乳腺癌（MCF7）和胶质母细胞瘤（U87）癌细胞系的细胞毒性活性。
从蛙皮中分离出一种产生抗生素的细菌。根据16S rDNA测序以及生化和形态学特征对该细菌进行鉴定。通过纸片扩散法和MIC法检测培养上清液的抗菌活性。通过超声处理制备细菌的细胞质和细胞壁提取物。然后使用SDS-PAGE检测它们的蛋白质含量。用不同浓度的细胞质和细胞壁提取物处理癌细胞系。在24小时和48小时间隔通过MTT法评估细胞毒性作用。最后，使用SPSS分析结果。
分离出的细菌被鉴定为一种新菌株。MIC和纸片扩散法表明该细菌的抗菌活性具有广谱性。MTT法显示，在暴露24 - 48小时后，U87和MCF7细胞的IC50值分别为30μg/ml和20μg/ml。
该细菌的细胞质提取物具有抗癌潜力，可作为癌症治疗中的替代或补充候选物。建议进一步进行体内和机制研究以证实其生物学活性。
