Isolation and identification of the bacterium producing antitumor and antimicrobial compounds derived from Iranian swamp frog skin.

BACKGROUND AND OBJECTIVES

Cancer incidence and recurrence, antibiotic resistance, and overuse of antibiotics have become a global concern. The purpose of this study was to identify and isolate bacteria from the skin of the , Iranian swamp frog, which has produced antimicrobial compounds, and investigate its cytotoxic activity on the breast (MCF7) and glioblastoma (U87) cancer cell line.

MATERIALS AND METHODS

An antibiotic-producing bacterium was isolated from the frog skin. The bacterium was identified based on 16S rDNA sequencing and biochemical and morphological characteristics. Antimicrobial activity of the culture supernatant was examined by disc diffusion and MIC methods. Cytoplasmic and cell wall extracts of bacteria were prepared by sonication. SDS-PAGE was then used to examine protein contents of them. The cancer cell lines were treated with cytoplasmic and cell wall extracts at different concentrations. The effects of cytotoxicity were assessed by MTT assay at 24 and 48 h intervals. Finally, the results were analyzed by SPSS.

RESULTS

The isolated bacterium was identified as a new strain of . MIC and disc diffusion methods showed that the antimicrobial activity was broad spectrum. MTT assay showed IC50 values 30 μg/ml and 20 μg/ml for U87 and MCF7 cells after 24-48 h exposure, respectively.

CONCLUSION

The cytoplasmic extracts of has anticancer potential and can be used as an alternative or complementary candidate in the treatment of cancer. Further and mechanistic studies are suggested to confirm the biological activities.