Fink Christine, Von Frieling Jakob, Knop Mirjam, Roeder Thomas
Molecular Physiology, Zoological Institute, CAU Kiel, Kiel, Germany.
Bio Protoc. 2017 Sep 20;7(18):e2547. doi: 10.21769/BioProtoc.2547.
Fecal sampling is a non-invasive method which raises the possibility to study the development and the changes in the microbial community throughout different time points of a fly population or throughout different treatments. This method allows precise manipulation to trigger the fly's physiology by nutritional interventions, bacterial infections or other stressors. As in most other animals, the intestinal microbiota is essential for a healthy fly-life. Because only harbors a relative simple bacterial community with a small variety of round about 8 to 10 different species, it is rather easy to build up the microbial community and to investigate microbial changes after treatment. Another positive effect using the fly's feces is that bacteria that are not part of the intestinal microbiome, for example , can be excluded directly from the analysis because they are not excreted. Using this method, the generated datasets may reflect a good paradigm to study microbiome associated diseases in a simple fly model or furthermore, to test drugs in a high-throughput approach.
粪便采样是一种非侵入性方法，它增加了在果蝇群体的不同时间点或不同处理过程中研究微生物群落发育和变化的可能性。这种方法允许通过营养干预、细菌感染或其他应激源进行精确操作来触发果蝇的生理反应。与大多数其他动物一样，肠道微生物群对果蝇的健康生活至关重要。由于果蝇仅拥有相对简单的细菌群落，大约有8到10种不同种类，因此建立微生物群落并研究处理后的微生物变化相当容易。使用果蝇粪便的另一个积极作用是，例如，不属于肠道微生物组的细菌可以直接从分析中排除，因为它们不会被排泄出来。使用这种方法，生成的数据集可能为在简单的果蝇模型中研究微生物组相关疾病，或者进一步以高通量方法测试药物提供一个很好的范例。
