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用于监测蒽环类药物进入哺乳动物细胞的摄取分析

Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells.

作者信息

Brosseau Nicolas, Andreev Emil, Ramotar Dindial

机构信息

Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Quebec, Canada.

出版信息

Bio Protoc. 2017 Sep 20;7(18):e2555. doi: 10.21769/BioProtoc.2555.

DOI:10.21769/BioProtoc.2555
PMID:34541201
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8413657/
Abstract

Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.

摘要

蒽环类药物，如阿霉素和柔红霉素，是能自发荧光的DNA损伤剂，可在细胞中轻易检测到。在此，我们开发了合适的检测方法来定量和定位哺乳动物细胞中的柔红霉素。这些检测方法可用于识别参与蒽环类药物摄取的成分。

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Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells.用于监测蒽环类药物进入哺乳动物细胞的摄取分析
Bio Protoc. 2017 Sep 20;7(18):e2555. doi: 10.21769/BioProtoc.2555.
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Complementation of the Yeast Model System Reveals that Caenorhabditis elegans OCT-1 Is a Functional Transporter of Anthracyclines.酵母模型系统的互补作用表明秀丽隐杆线虫OCT-1是蒽环类药物的功能性转运体。
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Cellular uptake, cytotoxicity, and transport kinetics of anthracyclines in human sensitive and multidrug-resistant K562 cells.蒽环类药物在人敏感及多药耐药K562细胞中的细胞摄取、细胞毒性及转运动力学
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The mechanism for anthracycline-induced inhibition of collagen biosynthesis.蒽环类药物诱导胶原蛋白生物合成抑制的机制。
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The antitumor anthracyclines doxorubicin and daunorubicin do not inhibit cell growth through the formation of iron-mediated reactive oxygen species.抗肿瘤蒽环类药物阿霉素和柔红霉素并非通过形成铁介导的活性氧来抑制细胞生长。
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Comparison of the interaction of doxorubicin, daunorubicin, idarubicin and idarubicinol with large unilamellar vesicles. Circular dichroism study.阿霉素、柔红霉素、伊达比星及伊达比星醇与大单层囊泡相互作用的比较。圆二色性研究。
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Activation of clinically used anthracyclines by the formaldehyde-releasing prodrug pivaloyloxymethyl butyrate.通过释放甲醛的前药丁酸匹伐酯激活临床使用的蒽环类药物。
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A phosphotyrosine switch regulates organic cation transporters.磷酸酪氨酸开关调节有机阳离子转运体。
Nat Commun. 2016 Mar 16;7:10880. doi: 10.1038/ncomms10880.
2
The human organic cation transporter OCT1 mediates high affinity uptake of the anticancer drug daunorubicin.人类有机阳离子转运体OCT1介导抗癌药物柔红霉素的高亲和力摄取。
Sci Rep. 2016 Feb 10;6:20508. doi: 10.1038/srep20508.
3
Anthracyclines potentiate anti-tumor immunity: A new opportunity for chemoimmunotherapy.蒽环类药物增强抗肿瘤免疫：化疗免疫治疗的新机会。
Cancer Lett. 2015 Dec 28;369(2):331-5. doi: 10.1016/j.canlet.2015.10.002. Epub 2015 Oct 11.
4
A Call for Systematic Research on Solute Carriers.呼吁对溶质载体进行系统研究。
Cell. 2015 Jul 30;162(3):478-87. doi: 10.1016/j.cell.2015.07.022.
5
Two repeated low doses of doxorubicin are more effective than a single high dose against tumors overexpressing P-glycoprotein.两种重复的低剂量阿霉素比单次高剂量更能有效对抗过度表达 P-糖蛋白的肿瘤。
Cancer Lett. 2015 May 1;360(2):219-26. doi: 10.1016/j.canlet.2015.02.008. Epub 2015 Feb 11.
6
Dexrazoxane may prevent doxorubicin-induced DNA damage via depleting both topoisomerase II isoforms.右丙亚胺可能通过消耗两种拓扑异构酶II同工型来预防阿霉素诱导的DNA损伤。
BMC Cancer. 2014 Nov 18;14:842. doi: 10.1186/1471-2407-14-842.
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Glycosylation of the OCTN2 carnitine transporter: study of natural mutations identified in patients with primary carnitine deficiency.OCTN2肉碱转运体的糖基化：对原发性肉碱缺乏症患者中鉴定出的自然突变的研究。
Biochim Biophys Acta. 2011 Mar;1812(3):312-20. doi: 10.1016/j.bbadis.2010.11.007. Epub 2010 Nov 29.
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The human carnitine transporter SLC22A16 mediates high affinity uptake of the anticancer polyamine analogue bleomycin-A5.人肉碱转运蛋白 SLC22A16 介导了抗癌多胺类似物博来霉素 A5 的高亲和力摄取。
J Biol Chem. 2010 Feb 26;285(9):6275-84. doi: 10.1074/jbc.M109.046151. Epub 2009 Dec 25.
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