用于监测蒽环类药物进入哺乳动物细胞的摄取分析
Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells.
作者信息
Brosseau Nicolas, Andreev Emil, Ramotar Dindial
机构信息
Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Quebec, Canada.
出版信息
Bio Protoc. 2017 Sep 20;7(18):e2555. doi: 10.21769/BioProtoc.2555.
Abstract
Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.
摘要
蒽环类药物，如阿霉素和柔红霉素，是能自发荧光的DNA损伤剂，可在细胞中轻易检测到。在此，我们开发了合适的检测方法来定量和定位哺乳动物细胞中的柔红霉素。这些检测方法可用于识别参与蒽环类药物摄取的成分。
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