Nawrot-Esposito Marie-Paule, Loudhaief Rihab, Gallet Armel
Universite Cote d'Azur, CNRS, INRA, ISA, France.
Institut Sophia Agrobiotech, UMR CNRS 7254/INRA 1355/UNS, 400 route des Chappes, BP 167, 06903 Sophia Antipolis Cedex, France.
Bio Protoc. 2017 Sep 20;7(18):e2560. doi: 10.21769/BioProtoc.2560.
The intestine is a central organ required for the digestion of food, the absorption of nutrients and for fighting against aggressors ingested along with the food. Impairment of gut physiology following mucosal damages impacts its digestive capacities that consequently will affect growth, wellbeing or even survival of the individual. Hence, the assessment of intestinal functions encompasses, among others, the monitoring of its integrity, its cellular renewing, its immune defenses, the production of enteroendocrine hormones and its digestive capacities. Here, we describe in detail how to assess the activity of the proteases secreted in the intestinal lumen of adult flies. This method can also be used for larval intestines. The present protocol is adapted and improved from the Sigma-Aldrich's protocol proposed in the 'Protease Fluorescent Detection Kit' (Product code PF0100).
肠道是食物消化、营养吸收以及抵御随食物摄入的病原体所必需的核心器官。黏膜损伤后肠道生理功能受损会影响其消化能力，进而影响个体的生长、健康甚至生存。因此，肠道功能评估包括监测其完整性、细胞更新、免疫防御、肠内分泌激素的产生及其消化能力等。在此，我们详细描述如何评估成年果蝇肠腔内分泌的蛋白酶的活性。该方法也可用于幼虫肠道。本方案是根据西格玛奥德里奇公司在“蛋白酶荧光检测试剂盒”（产品代码PF0100）中提出的方案改编和改进的。
