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编码δ-内毒素的苏云金芽孢杆菌质粒在苏云金芽孢杆菌菌株和蜡样芽孢杆菌菌株间的转移。

Transfer of Bacillus thuringiensis plasmids coding for delta-endotoxin among strains of B. thuringiensis and B. cereus.

作者信息

González J M, Brown B J, Carlton B C

出版信息

Proc Natl Acad Sci U S A. 1982 Nov;79(22):6951-5. doi: 10.1073/pnas.79.22.6951.

DOI:10.1073/pnas.79.22.6951
PMID:6294667
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC347252/
Abstract

The recently discovered high-frequency transfer of plasmids between strains of Bacillus thuringiensis was used to study the genetic relationship between plasmids and production of the insecticidal delta-endotoxin crystal. Three strains of B. thuringiensis transmitted the Cry+ (crystal-producing) phenotype to Cry- (acrystalliferous) B. thuringiensis recipients. Agarose gel electrophoresis showed that one specific plasmid from each donor strain was always present in Cry+ "transcipients." The size of the transmissible crystal-coding plasmid varied with the donor strain, being 75 MDal (megadaltons) in size in HD-2, 50 MDal in HD-73, and 44 MDal in HD-263. Immunological analysis showed the Cry+ transcipients to be hybrid strains, having flagella of the recipient serotype and crystals of the donor serotype. These results demonstrate that the structural genes for the delta-endotoxin are plasmid borne. Crystal-coding plasmids also transferred into two strains of the related species Bacillus cereus and yielded transcipients that produced crystals of the same antigenicity as the donor strain.

摘要

最近发现的苏云金芽孢杆菌菌株间质粒的高频转移被用于研究质粒与杀虫δ-内毒素晶体产生之间的遗传关系。三株苏云金芽孢杆菌将Cry+（产生晶体的）表型传递给了Cry-（不产晶体的）苏云金芽孢杆菌受体菌。琼脂糖凝胶电泳显示，来自每个供体菌株的一种特定质粒总是存在于Cry+“转导子”中。可传递的晶体编码质粒的大小因供体菌株而异，HD-2中为75兆道尔顿（MDal），HD-73中为50 MDal，HD-263中为44 MDal。免疫分析表明，Cry+转导子是杂交菌株，具有受体血清型的鞭毛和供体血清型的晶体。这些结果表明，δ-内毒素的结构基因由质粒携带。晶体编码质粒也转移到了相关物种蜡状芽孢杆菌的两株菌株中，并产生了与供体菌株具有相同抗原性晶体的转导子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/3a6b8d156fcc/pnas00461-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/668e183fd63d/pnas00461-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/fcab031d01ab/pnas00461-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/eef4d10689f5/pnas00461-0224-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/3a6b8d156fcc/pnas00461-0225-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/668e183fd63d/pnas00461-0222-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/fcab031d01ab/pnas00461-0224-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/eef4d10689f5/pnas00461-0224-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4988/347252/3a6b8d156fcc/pnas00461-0225-a.jpg

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