硫酸乙酰肝素的鉴定与表征：方法I. 通过核磁共振分析鉴定硫酸乙酰肝素
Heparan Sulfate Identification and Characterisation: Method I.Heparan Sulfate Identification by NMR Analysis.
作者信息
Carnachan Susan M, Hinkley Simon F R
机构信息
The Ferrier Research Institute, Victoria University of Wellington, Lower Hutt, New Zealand.
出版信息
Bio Protoc. 2017 Apr 5;7(7):e2196. doi: 10.21769/BioProtoc.2196.
Abstract
Heparin and heparan sulfate (HS) may be purified from complex biological matrices and are often isolated in sub-milligram quantities but not unequivocally identified by spectroscopic means. This protocol details a methodology to rapidly assess the gross compositional features and approximate purity of HS by H nuclear magnetic resonance. A complimentary method for identification and characterisation of heparan sulfate can be found at Carnachan and Hinkley (2017).
摘要
肝素和硫酸乙酰肝素（HS）可从复杂的生物基质中纯化得到，通常以亚毫克量分离，但无法通过光谱手段明确鉴定。本方案详细介绍了一种通过氢核磁共振快速评估HS总体组成特征和近似纯度的方法。硫酸乙酰肝素鉴定和表征的补充方法可参考Carnachan和Hinkley（2017年）的文献。
相似文献
1
Heparan Sulfate Identification and Characterisation: Method I.Heparan Sulfate Identification by NMR Analysis.硫酸乙酰肝素的鉴定与表征：方法I. 通过核磁共振分析鉴定硫酸乙酰肝素
Bio Protoc. 2017 Apr 5;7(7):e2196. doi: 10.21769/BioProtoc.2196.
2
Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition.硫酸乙酰肝素的鉴定与表征：方法二。酶促解聚及SAX-HPLC分析以确定二糖组成。
Bio Protoc. 2017 Apr 5;7(7):e2197. doi: 10.21769/BioProtoc.2197.
3
Structural characterization of human liver heparan sulfate.人肝脏硫酸乙酰肝素的结构表征
Biochim Biophys Acta. 2005 Jan 18;1721(1-3):1-8. doi: 10.1016/j.bbagen.2004.09.007. Epub 2004 Nov 11.
4
(1)H nuclear magnetic resonance spectroscopic analysis for determination of glucuronic and iduronic acids in dermatan sulfate, heparin, and heparan sulfate.（1）用于测定硫酸皮肤素、肝素和硫酸乙酰肝素中葡萄糖醛酸和艾杜糖醛酸的氢核磁共振光谱分析。
Anal Biochem. 2001 Oct 1;297(1):42-51. doi: 10.1006/abio.2001.5296.
5
Preparation of Isotope-Enriched Heparan Sulfate Precursors for Structural Biology Studies.用于结构生物学研究的同位素标记硫酸乙酰肝素前体的制备。
Methods Mol Biol. 2022;2303:341-347. doi: 10.1007/978-1-0716-1398-6_28.
6
Disaccharide compositional analysis of heparin and heparan sulfate using capillary zone electrophoresis.使用毛细管区带电泳对肝素和硫酸乙酰肝素进行二糖组成分析。
Anal Biochem. 1991 Dec;199(2):249-55. doi: 10.1016/0003-2697(91)90098-e.
7
Chemoenzymatic synthesis of heparan sulfate and heparin oligosaccharides and NMR analysis: paving the way to a diverse library for glycobiologists.硫酸乙酰肝素和肝素寡糖的化学酶法合成及核磁共振分析：为糖生物学家打造多样化文库铺平道路。
Chem Sci. 2017 Dec 1;8(12):7932-7940. doi: 10.1039/c7sc03541a. Epub 2017 Sep 21.
8
A novel computational approach to integrate NMR spectroscopy and capillary electrophoresis for structure assignment of heparin and heparan sulfate oligosaccharides.一种整合核磁共振光谱和毛细管电泳用于肝素及硫酸乙酰肝素寡糖结构鉴定的新型计算方法。
Glycobiology. 2002 Nov;12(11):713-9. doi: 10.1093/glycob/cwf084.
9
Identification and characterization of heparin/heparan sulfate binding domains of the endoglycosidase heparanase.内切糖苷酶乙酰肝素酶的肝素/硫酸乙酰肝素结合结构域的鉴定与表征
J Biol Chem. 2005 May 27;280(21):20457-66. doi: 10.1074/jbc.M414546200. Epub 2005 Mar 10.
10
GAG-ID: Heparan Sulfate (HS) and Heparin Glycosaminoglycan High-Throughput Identification Software.GAG-ID：硫酸乙酰肝素（HS）和肝素糖胺聚糖高通量鉴定软件。
Mol Cell Proteomics. 2015 Jun;14(6):1720-30. doi: 10.1074/mcp.M114.045856. Epub 2015 Apr 17.
引用本文的文献
1
Sulfonate Hydrogel-siRNA Conjugate Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells by Controlled Gene Silencing and Activation of BMP Signaling.磺酸基水凝胶-siRNA 缀合物通过控制基因沉默和激活 BMP 信号促进间充质干细胞的成骨分化。
ACS Appl Bio Mater. 2021 Jun 21;4(6):5189-5200. doi: 10.1021/acsabm.1c00369. Epub 2021 May 27.
2
Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition.硫酸乙酰肝素的鉴定与表征：方法二。酶促解聚及SAX-HPLC分析以确定二糖组成。
Bio Protoc. 2017 Apr 5;7(7):e2197. doi: 10.21769/BioProtoc.2197.
3
Analysis of the Glycosaminoglycan Chains of Proteoglycans.糖胺聚糖链分析蛋白聚糖。
J Histochem Cytochem. 2021 Feb;69(2):121-135. doi: 10.1369/0022155420937154. Epub 2020 Jul 6.
4
A Fluorescent Probe for Glycosaminoglycans Applied to the Detection of Dermatan Sulfate by a Mix-and-Read Assay.一种用于糖胺聚糖的荧光探针，通过混合读取法用于检测硫酸皮肤素。
Molecules. 2017 May 9;22(5):768. doi: 10.3390/molecules22050768.
本文引用的文献
1
Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition.硫酸乙酰肝素的鉴定与表征：方法二。酶促解聚及SAX-HPLC分析以确定二糖组成。
Bio Protoc. 2017 Apr 5;7(7):e2197. doi: 10.21769/BioProtoc.2197.
2
Determining the extent of heparan sulfate depolymerisation following heparin lyase treatment.确定肝素酶处理后硫酸乙酰肝素解聚的程度。
Carbohydr Polym. 2016 Nov 5;152:592-597. doi: 10.1016/j.carbpol.2016.07.024. Epub 2016 Jul 7.
3
Assay of possible economically motivated additives or native impurities levels in heparin by 1H NMR, SAX-HPLC, and anticoagulation time approaches.采用 1H NMR、SAX-HPLC 和抗凝时间测定法测定肝素中可能存在的具有经济动机的添加剂或天然杂质水平。
J Pharm Biomed Anal. 2010 Sep 5;52(5):656-64. doi: 10.1016/j.jpba.2010.02.019. Epub 2010 Feb 20.
4
Lessons learned from the contamination of heparin.从肝素污染事件中吸取的教训。
Nat Prod Rep. 2009 Mar;26(3):313-21. doi: 10.1039/b819896a. Epub 2009 Jan 19.
5
Oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events.过度硫酸化硫酸软骨素是肝素中的一种污染物，与不良临床事件相关。
Nat Biotechnol. 2008 Jun;26(6):669-75. doi: 10.1038/nbt1407. Epub 2008 Apr 23.
6
Complex glycosaminoglycans: profiling substitution patterns by two-dimensional nuclear magnetic resonance spectroscopy.复合糖胺聚糖：通过二维核磁共振光谱分析取代模式
Anal Biochem. 2005 Feb 1;337(1):35-47. doi: 10.1016/j.ab.2004.10.012.
7
High-specific-activity 35S-labeled heparan sulfate prepared from cultured cells.从培养细胞中制备的高比活度35S标记硫酸乙酰肝素。
Methods Mol Biol. 2001;171:79-89. doi: 10.1385/1-59259-209-0:079.
Zotero 插件