硫酸乙酰肝素的鉴定与表征：方法二。酶促解聚及SAX-HPLC分析以确定二糖组成。
Heparan Sulfate Identification and Characterisation: Method II. Enzymatic Depolymerisation and SAX-HPLC Analysis to Determine Disaccharide Composition.
作者信息
Carnachan Susan M, Hinkley Simon F R
机构信息
The Ferrier Research Institute, Victoria University of Wellington, Lower Hutt, New Zealand.
出版信息
Bio Protoc. 2017 Apr 5;7(7):e2197. doi: 10.21769/BioProtoc.2197.
Abstract
Heparan sulfate (HS) is purified from complex matrices and often not fully characterised to validate its assignment. The characterisation of heparins and heparan sulfates through enzymatic depolymerisation and subsequent strong anion-exchange high performance liquid chromatography (SAX-HPLC) analysis and quantitation of the resulting disaccharides is a critical tool for assessing the structural composition of this class of compound. This protocol details a methodology to reproducibly determine the disaccharide composition of heparan sulfate by enzymatic depolymerisation and SAX-HPLC analysis. A complementary method for identification and characterisation of heparan sulfate can be found at Carnachan and Hinkley (2017).
摘要
硫酸乙酰肝素（HS）是从复杂基质中纯化得到的，且常常没有经过充分表征以验证其归属。通过酶促解聚以及随后的强阴离子交换高效液相色谱（SAX-HPLC）分析和对所得二糖的定量来表征肝素和硫酸乙酰肝素，是评估这类化合物结构组成的关键工具。本方案详细介绍了一种通过酶促解聚和SAX-HPLC分析可重复测定硫酸乙酰肝素二糖组成的方法。在Carnachan和Hinkley（2017年）中可以找到一种用于鉴定和表征硫酸乙酰肝素的补充方法。
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