用于鉴定 miRNA 靶标的生物素化微小 RNA 下拉分析
Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets.
作者信息
Phatak Pornima, Donahue James M
机构信息
Department of Surgery, University of Maryland School of Medicine, Baltimore, USA.
Baltimore Veterans Affairs Medical Center, Baltimore, USA.
出版信息
Bio Protoc. 2017 May 5;7(9):e2253. doi: 10.21769/BioProtoc.2253.
Abstract
microRNA (miRNA) directly associates with its target transcripts (mRNA). This protocol describes a method for detection of direct interaction between miRNA and mRNA. The result of interaction helps screening the specific target mRNAs for a miRNA.
摘要
微小RNA（miRNA）直接与其靶转录本（mRNA）相互作用。本方案描述了一种检测miRNA与mRNA之间直接相互作用的方法。相互作用的结果有助于筛选miRNA的特定靶mRNA。
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