• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

间充质基质细胞通过 miR-29c 和 miR-129 的细胞间转移促进肺纤维化的解决。

Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer.

机构信息

Institute for Regenerative Medicine, Medical Research and Education Center, Lomonosov Moscow State University, Moscow, Russian Federation.

Faculty of Medicine, Lomonosov Moscow State University, Moscow, Russian Federation.

出版信息

Exp Mol Med. 2023 Jul;55(7):1399-1412. doi: 10.1038/s12276-023-01017-w. Epub 2023 Jul 3.

DOI:10.1038/s12276-023-01017-w
PMID:37394579
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10393964/
Abstract

To date, pulmonary fibrosis remains an unmet medical need. In this study, we evaluated the potency of mesenchymal stromal cell (MSC) secretome components to prevent pulmonary fibrosis development and facilitate fibrosis resolution. Surprisingly, the intratracheal application of extracellular vesicles (MSC-EVs) or the vesicle-depleted secretome fraction (MSC-SF) was not able to prevent lung fibrosis when applied immediately after the injury caused by bleomycin instillation in mice. However, MSC-EV administration induced the resolution of established pulmonary fibrosis, whereas the vesicle-depleted fraction did not. The application of MSC-EVs caused a decrease in the numbers of myofibroblasts and FAPa progenitors without affecting their apoptosis. Such a decrease likely occurred due to their dedifferentiation caused by microRNA (miR) transfer by MSC-EVs. Using a murine model of bleomycin-induced pulmonary fibrosis, we confirmed the contribution of specific miRs (miR-29c and miR-129) to the antifibrotic effect of MSC-EVs. Our study provides novel insights into possible antifibrotic therapy based on the use of the vesicle-enriched fraction of the MSC secretome.

摘要

迄今为止，肺纤维化仍然是一种未满足的医学需求。在这项研究中，我们评估了间充质基质细胞（MSC）分泌组成分预防肺纤维化发展和促进纤维化消退的效力。令人惊讶的是，当在博莱霉素滴注引起的损伤后立即应用于小鼠时，细胞外囊泡（MSC-EVs）或囊泡耗尽的分泌组部分（MSC-SF）的气管内应用不能预防肺纤维化。然而，MSC-EV 的给药诱导了已建立的肺纤维化的消退，而囊泡耗尽的部分则没有。MSC-EV 的应用导致肌成纤维细胞和 FAPa 祖细胞数量减少，而不影响其凋亡。这种减少可能是由于 MSC-EVs 通过 microRNA（miR）转移导致其去分化。使用博莱霉素诱导的肺纤维化的小鼠模型，我们证实了特定的 miR（miR-29c 和 miR-129）对 MSC-EVs 的抗纤维化作用的贡献。我们的研究为基于使用 MSC 分泌组的富含囊泡部分的可能的抗纤维化治疗提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/f1eaaca02857/12276_2023_1017_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/708c15579b96/12276_2023_1017_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/c5f10dd559df/12276_2023_1017_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/3320d7ed4037/12276_2023_1017_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ea476162a18a/12276_2023_1017_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ef656d834536/12276_2023_1017_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/f1eaaca02857/12276_2023_1017_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/708c15579b96/12276_2023_1017_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/c5f10dd559df/12276_2023_1017_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/3320d7ed4037/12276_2023_1017_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ea476162a18a/12276_2023_1017_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ef656d834536/12276_2023_1017_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/f1eaaca02857/12276_2023_1017_Fig6_HTML.jpg

相似文献

1
Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer.间充质基质细胞通过 miR-29c 和 miR-129 的细胞间转移促进肺纤维化的解决。
Exp Mol Med. 2023 Jul;55(7):1399-1412. doi: 10.1038/s12276-023-01017-w. Epub 2023 Jul 3.
2
Secretome of Mesenchymal Stromal Cells Prevents Myofibroblasts Differentiation by Transferring Fibrosis-Associated microRNAs within Extracellular Vesicles.间充质基质细胞的分泌组通过细胞外囊泡内转移纤维化相关 microRNAs 防止肌成纤维细胞分化。
Cells. 2020 May 20;9(5):1272. doi: 10.3390/cells9051272.
3
Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer's disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway.间质干细胞衍生的细胞外囊泡通过 microRNA-29c-3p/BACE1 轴和 Wnt/β-连环蛋白通路改善大鼠阿尔茨海默病模型。
Aging (Albany NY). 2021 Jun 4;13(11):15285-15306. doi: 10.18632/aging.203088.
4
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles alleviated silica induced lung inflammation and fibrosis in mice via circPWWP2A/miR-223-3p/NLRP3 axis.人脐带间充质干细胞来源的细胞外囊泡通过circPWWP2A/miR-223-3p/NLRP3轴减轻二氧化硅诱导的小鼠肺部炎症和纤维化。
Ecotoxicol Environ Saf. 2023 Feb;251:114537. doi: 10.1016/j.ecoenv.2023.114537. Epub 2023 Jan 14.
5
Protective effect of miRNA-containing extracellular vesicles derived from mesenchymal stromal cells of old rats on renal function in chronic kidney disease.老年大鼠间充质基质细胞来源的含 miRNA 的细胞外囊泡对慢性肾脏病肾功能的保护作用。
Stem Cell Res Ther. 2020 Jul 8;11(1):274. doi: 10.1186/s13287-020-01792-7.
6
Sodium hydrosulfide and bone marrow derived mesenchymal stem cells combined therapy for bleomycin induced pulmonary fibrosis in rats: Implication of micro RNA-21 and Lnc GAS5.硫化氢钠和骨髓间充质干细胞联合治疗博来霉素诱导的大鼠肺纤维化：微小 RNA-21 和长链非编码 RNA GAS5 的作用。
Life Sci. 2022 Nov 15;309:120988. doi: 10.1016/j.lfs.2022.120988. Epub 2022 Sep 22.
7
Human bone marrow-derived mesenchymal stromal cells cultured in serum-free media demonstrate enhanced antifibrotic abilities via prolonged survival and robust regulatory T cell induction in murine bleomycin-induced pulmonary fibrosis.人骨髓间充质基质细胞在无血清培养基中培养，通过延长存活时间和强烈诱导调节性 T 细胞，在小鼠博来霉素诱导的肺纤维化中显示出增强的抗纤维化能力。
Stem Cell Res Ther. 2021 Sep 16;12(1):506. doi: 10.1186/s13287-021-02574-5.
8
Extracellular vesicles derived from umbilical cord mesenchymal stromal cells alleviate pulmonary fibrosis by means of transforming growth factor-β signaling inhibition.脐带间充质干细胞来源的细胞外囊泡通过抑制转化生长因子-β信号通路缓解肺纤维化。
Stem Cell Res Ther. 2021 Apr 12;12(1):230. doi: 10.1186/s13287-021-02296-8.
9
Reprogrammed lung epithelial cells by decrease of miR-451a in extracellular vesicles contribute to aggravation of pulmonary fibrosis.通过细胞外囊泡中 miR-451a 的减少重编程肺上皮细胞有助于加重肺纤维化。
Cell Biol Toxicol. 2022 Oct;38(5):725-740. doi: 10.1007/s10565-021-09626-9. Epub 2021 Aug 30.
10
Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Dendritic Cell Maturation and Function.间质基质细胞衍生的细胞外囊泡可减轻树突状细胞的成熟和功能。
Front Immunol. 2018 Nov 9;9:2538. doi: 10.3389/fimmu.2018.02538. eCollection 2018.

引用本文的文献

1
Acomys cahirinus develop lung stroma distortion but not fibrosis after bleomycin-induced injury.埃及刺毛鼠在博来霉素诱导损伤后会出现肺基质变形，但不会出现纤维化。
Inflamm Regen. 2025 Aug 29;45(1):27. doi: 10.1186/s41232-025-00391-4.
2
MSC-Derived Extracellular Vesicles: Roles and Molecular Mechanisms for Tissue Repair.间充质干细胞衍生的细胞外囊泡：组织修复中的作用和分子机制
Int J Nanomedicine. 2025 Jun 21;20:7953-7974. doi: 10.2147/IJN.S525394. eCollection 2025.
3
The Use of Extracellular Vesicles as a Promising Therapeutic Approach for Pulmonary Diseases.

本文引用的文献

1
The Role of Extracellular Vesicles in Idiopathic Pulmonary Fibrosis Progression: An Approach on Their Therapeutics Potential.细胞外囊泡在特发性肺纤维化进展中的作用：对其治疗潜力的探讨。
Cells. 2022 Feb 11;11(4):630. doi: 10.3390/cells11040630.
2
Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets.用于鉴定 miRNA 靶标的生物素化微小 RNA 下拉分析
Bio Protoc. 2017 May 5;7(9):e2253. doi: 10.21769/BioProtoc.2253.
3
Macrophages in lung fibrosis.肺纤维化中的巨噬细胞。
细胞外囊泡作为肺部疾病一种有前景的治疗方法的应用。
Health Sci Rep. 2025 Jun 11;8(6):e70853. doi: 10.1002/hsr2.70853. eCollection 2025 Jun.
4
Advances in locally administered nucleic acid therapeutics.局部给药核酸疗法的进展。
Bioact Mater. 2025 Mar 10;49:218-254. doi: 10.1016/j.bioactmat.2025.02.043. eCollection 2025 Jul.
5
Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Pathogenesis, Biomarkers and Innovative Therapeutic Strategies.特发性肺纤维化中的细胞外囊泡：发病机制、生物标志物及创新治疗策略
Int J Nanomedicine. 2024 Nov 25;19:12593-12614. doi: 10.2147/IJN.S491335. eCollection 2024.
6
Revisiting the role of MicroRNAs in the pathogenesis of idiopathic pulmonary fibrosis.重新审视微小RNA在特发性肺纤维化发病机制中的作用。
Front Cell Dev Biol. 2024 Oct 16;12:1470875. doi: 10.3389/fcell.2024.1470875. eCollection 2024.
7
The Role of MicroRNAs in Mesenchymal Stem Cell-Based Modulation of Pulmonary Fibrosis.微小 RNA 在间充质干细胞调控肺纤维化中的作用。
Cell Transplant. 2024 Jan-Dec;33:9636897241281026. doi: 10.1177/09636897241281026.
8
Therapeutic Application of Extracellular Vesicles Derived from Mesenchymal Stem Cells in Domestic Animals.间充质干细胞来源的细胞外囊泡在家畜中的治疗应用。
Animals (Basel). 2024 Jul 24;14(15):2147. doi: 10.3390/ani14152147.
9
Regulation of myofibroblast dedifferentiation in pulmonary fibrosis.肺纤维化中肌成纤维细胞去分化的调控。
Respir Res. 2024 Jul 18;25(1):284. doi: 10.1186/s12931-024-02898-9.
10
Mesenchymal stem cell secretome for regenerative medicine: Where do we stand?用于再生医学的间充质干细胞分泌组：我们目前的状况如何？
J Adv Res. 2025 Apr;70:103-124. doi: 10.1016/j.jare.2024.05.004. Epub 2024 May 9.
Int Immunol. 2021 Nov 25;33(12):665-671. doi: 10.1093/intimm/dxab040.
4
Exosomal MicroRNA-21 Promotes Keloid Fibroblast Proliferation and Collagen Production by Inhibiting Smad7.外泌体 MicroRNA-21 通过抑制 Smad7 促进瘢痕疙瘩成纤维细胞的增殖和胶原生成。
J Burn Care Res. 2021 Nov 24;42(6):1266-1274. doi: 10.1093/jbcr/irab116.
5
Myofibroblast dedifferentiation proceeds via distinct transcriptomic and phenotypic transitions.肌成纤维细胞去分化是通过不同的转录组和表型转变来进行的。
JCI Insight. 2021 Mar 22;6(6):144799. doi: 10.1172/jci.insight.144799.
6
Secretome of Mesenchymal Stromal Cells Prevents Myofibroblasts Differentiation by Transferring Fibrosis-Associated microRNAs within Extracellular Vesicles.间充质基质细胞的分泌组通过细胞外囊泡内转移纤维化相关 microRNAs 防止肌成纤维细胞分化。
Cells. 2020 May 20;9(5):1272. doi: 10.3390/cells9051272.
7
Extracellular vesicles derived from mesenchymal stem cells prevent skin fibrosis in the cGVHD mouse model by suppressing the activation of macrophages and B cells immune response.间充质干细胞来源的细胞外囊泡通过抑制巨噬细胞和 B 细胞免疫应答来预防 cGVHD 小鼠模型中的皮肤纤维化。
Int Immunopharmacol. 2020 Jul;84:106541. doi: 10.1016/j.intimp.2020.106541. Epub 2020 May 18.
8
Mechanisms underlying the protective effects of mesenchymal stem cell-based therapy.基于间充质干细胞治疗的保护作用的机制。
Cell Mol Life Sci. 2020 Jul;77(14):2771-2794. doi: 10.1007/s00018-020-03454-6. Epub 2020 Jan 21.
9
Anti-fibrotic mechanisms of exogenously-expanded mesenchymal stromal cells for fibrotic diseases.外源性扩增的间充质基质细胞治疗纤维化疾病的抗纤维化机制。
Semin Cell Dev Biol. 2020 May;101:87-103. doi: 10.1016/j.semcdb.2019.10.014. Epub 2019 Nov 19.
10
Mesenchymal stromal cell exosomes prevent and revert experimental pulmonary fibrosis through modulation of monocyte phenotypes.间质基质细胞外泌体通过调节单核细胞表型预防和逆转实验性肺纤维化。
JCI Insight. 2019 Nov 1;4(21):128060. doi: 10.1172/jci.insight.128060.