文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

间充质基质细胞通过 miR-29c 和 miR-129 的细胞间转移促进肺纤维化的解决。

Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer.

机构信息

Institute for Regenerative Medicine, Medical Research and Education Center, Lomonosov Moscow State University, Moscow, Russian Federation.

Faculty of Medicine, Lomonosov Moscow State University, Moscow, Russian Federation.

出版信息

Exp Mol Med. 2023 Jul;55(7):1399-1412. doi: 10.1038/s12276-023-01017-w. Epub 2023 Jul 3.

DOI:10.1038/s12276-023-01017-w
PMID:37394579
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10393964/
Abstract

To date, pulmonary fibrosis remains an unmet medical need. In this study, we evaluated the potency of mesenchymal stromal cell (MSC) secretome components to prevent pulmonary fibrosis development and facilitate fibrosis resolution. Surprisingly, the intratracheal application of extracellular vesicles (MSC-EVs) or the vesicle-depleted secretome fraction (MSC-SF) was not able to prevent lung fibrosis when applied immediately after the injury caused by bleomycin instillation in mice. However, MSC-EV administration induced the resolution of established pulmonary fibrosis, whereas the vesicle-depleted fraction did not. The application of MSC-EVs caused a decrease in the numbers of myofibroblasts and FAPa progenitors without affecting their apoptosis. Such a decrease likely occurred due to their dedifferentiation caused by microRNA (miR) transfer by MSC-EVs. Using a murine model of bleomycin-induced pulmonary fibrosis, we confirmed the contribution of specific miRs (miR-29c and miR-129) to the antifibrotic effect of MSC-EVs. Our study provides novel insights into possible antifibrotic therapy based on the use of the vesicle-enriched fraction of the MSC secretome.

摘要

迄今为止，肺纤维化仍然是一种未满足的医学需求。在这项研究中，我们评估了间充质基质细胞（MSC）分泌组成分预防肺纤维化发展和促进纤维化消退的效力。令人惊讶的是，当在博莱霉素滴注引起的损伤后立即应用于小鼠时，细胞外囊泡（MSC-EVs）或囊泡耗尽的分泌组部分（MSC-SF）的气管内应用不能预防肺纤维化。然而，MSC-EV 的给药诱导了已建立的肺纤维化的消退，而囊泡耗尽的部分则没有。MSC-EV 的应用导致肌成纤维细胞和 FAPa 祖细胞数量减少，而不影响其凋亡。这种减少可能是由于 MSC-EVs 通过 microRNA（miR）转移导致其去分化。使用博莱霉素诱导的肺纤维化的小鼠模型，我们证实了特定的 miR（miR-29c 和 miR-129）对 MSC-EVs 的抗纤维化作用的贡献。我们的研究为基于使用 MSC 分泌组的富含囊泡部分的可能的抗纤维化治疗提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/f1eaaca02857/12276_2023_1017_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/708c15579b96/12276_2023_1017_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/c5f10dd559df/12276_2023_1017_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/3320d7ed4037/12276_2023_1017_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ea476162a18a/12276_2023_1017_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ef656d834536/12276_2023_1017_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/f1eaaca02857/12276_2023_1017_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/708c15579b96/12276_2023_1017_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/c5f10dd559df/12276_2023_1017_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/3320d7ed4037/12276_2023_1017_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ea476162a18a/12276_2023_1017_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/ef656d834536/12276_2023_1017_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99ff/10393964/f1eaaca02857/12276_2023_1017_Fig6_HTML.jpg

相似文献

[1]
Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer.

Exp Mol Med. 2023-7

[2]
Secretome of Mesenchymal Stromal Cells Prevents Myofibroblasts Differentiation by Transferring Fibrosis-Associated microRNAs within Extracellular Vesicles.

Cells. 2020-5-20

[3]
Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer's disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway.

Aging (Albany NY). 2021-6-4

[4]
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles alleviated silica induced lung inflammation and fibrosis in mice via circPWWP2A/miR-223-3p/NLRP3 axis.

Ecotoxicol Environ Saf. 2023-2

[5]
Protective effect of miRNA-containing extracellular vesicles derived from mesenchymal stromal cells of old rats on renal function in chronic kidney disease.

Stem Cell Res Ther. 2020-7-8

[6]
Sodium hydrosulfide and bone marrow derived mesenchymal stem cells combined therapy for bleomycin induced pulmonary fibrosis in rats: Implication of micro RNA-21 and Lnc GAS5.

Life Sci. 2022-11-15

[7]
Human bone marrow-derived mesenchymal stromal cells cultured in serum-free media demonstrate enhanced antifibrotic abilities via prolonged survival and robust regulatory T cell induction in murine bleomycin-induced pulmonary fibrosis.

Stem Cell Res Ther. 2021-9-16

[8]
Extracellular vesicles derived from umbilical cord mesenchymal stromal cells alleviate pulmonary fibrosis by means of transforming growth factor-β signaling inhibition.

Stem Cell Res Ther. 2021-4-12

[9]
Reprogrammed lung epithelial cells by decrease of miR-451a in extracellular vesicles contribute to aggravation of pulmonary fibrosis.

Cell Biol Toxicol. 2022-10

[10]
Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Dendritic Cell Maturation and Function.

Front Immunol. 2018-11-9

引用本文的文献

[1]
Acomys cahirinus develop lung stroma distortion but not fibrosis after bleomycin-induced injury.

Inflamm Regen. 2025-8-29

[2]
MSC-Derived Extracellular Vesicles: Roles and Molecular Mechanisms for Tissue Repair.

Int J Nanomedicine. 2025-6-21

[3]
The Use of Extracellular Vesicles as a Promising Therapeutic Approach for Pulmonary Diseases.

Health Sci Rep. 2025-6-11

[4]
Advances in locally administered nucleic acid therapeutics.

Bioact Mater. 2025-3-10

[5]
Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Pathogenesis, Biomarkers and Innovative Therapeutic Strategies.

Int J Nanomedicine. 2024-11-25

[6]
Revisiting the role of MicroRNAs in the pathogenesis of idiopathic pulmonary fibrosis.

Front Cell Dev Biol. 2024-10-16

[7]
The Role of MicroRNAs in Mesenchymal Stem Cell-Based Modulation of Pulmonary Fibrosis.

Cell Transplant. 2024

[8]
Therapeutic Application of Extracellular Vesicles Derived from Mesenchymal Stem Cells in Domestic Animals.

Animals (Basel). 2024-7-24

[9]
Regulation of myofibroblast dedifferentiation in pulmonary fibrosis.

Respir Res. 2024-7-18

[10]
Mesenchymal stem cell secretome for regenerative medicine: Where do we stand?

J Adv Res. 2025-4

本文引用的文献

[1]
The Role of Extracellular Vesicles in Idiopathic Pulmonary Fibrosis Progression: An Approach on Their Therapeutics Potential.

Cells. 2022-2-11

[2]
Biotinylated Micro-RNA Pull Down Assay for Identifying miRNA Targets.

Bio Protoc. 2017-5-5

[3]
Macrophages in lung fibrosis.

Int Immunol. 2021-11-25

[4]
Exosomal MicroRNA-21 Promotes Keloid Fibroblast Proliferation and Collagen Production by Inhibiting Smad7.

J Burn Care Res. 2021-11-24

[5]
Myofibroblast dedifferentiation proceeds via distinct transcriptomic and phenotypic transitions.

JCI Insight. 2021-3-22

[6]
Secretome of Mesenchymal Stromal Cells Prevents Myofibroblasts Differentiation by Transferring Fibrosis-Associated microRNAs within Extracellular Vesicles.

Cells. 2020-5-20

[7]
Extracellular vesicles derived from mesenchymal stem cells prevent skin fibrosis in the cGVHD mouse model by suppressing the activation of macrophages and B cells immune response.

Int Immunopharmacol. 2020-7

[8]
Mechanisms underlying the protective effects of mesenchymal stem cell-based therapy.

Cell Mol Life Sci. 2020-1-21

[9]
Anti-fibrotic mechanisms of exogenously-expanded mesenchymal stromal cells for fibrotic diseases.

Semin Cell Dev Biol. 2020-5

[10]
Mesenchymal stromal cell exosomes prevent and revert experimental pulmonary fibrosis through modulation of monocyte phenotypes.

JCI Insight. 2019-11-1

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索