Department of Pharmacy, Guangdong Second Provincial General Hospital, Guangzhou, China.
State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.
Front Cell Infect Microbiol. 2022 Jul 7;12:904775. doi: 10.3389/fcimb.2022.904775. eCollection 2022.
Given the frequent emergence of drug-resistant influenza virus strains and new highly pathogenic influenza virus strains, there is an urgent need to identify new antiviral drugs and targets. We found that influenza A virus (IAV) infection caused a significant decrease of microRNA let-7 expression in host cells; that overexpression of let-7 increased interferon expression and effectively inhibit IAV infection; and that let-7 targets the 3'-untranslated region (UTR) of the ribosomal protein 16 () gene, decreasing its expression. Knocking down the expression of increased the expression of type I interferon and inhibited viral replication. The present study uncovered the regulatory effect of let-7b and let-7f on influenza A infection, which is a potential biomarker of IAV infection. In addition, let-7 may be a promising therapeutic agent against influenza A.
鉴于耐药性流感病毒株和新型高致病性流感病毒株的频繁出现，急需寻找新的抗病毒药物和靶标。我们发现流感病毒（IAV）感染可导致宿主细胞中 microRNA let-7 的表达显著下调；过表达 let-7 可增加干扰素的表达并有效抑制 IAV 感染；let-7 靶向核糖体蛋白 16（）基因的 3'-非翻译区（UTR），降低其表达。敲低的表达可增加 I 型干扰素的表达并抑制病毒复制。本研究揭示了 let-7b 和 let-7f 对流感 A 感染的调节作用，它们可能是 IAV 感染的潜在生物标志物。此外，let-7 可能是一种有前途的抗流感 A 治疗药物。
