Ishida Juliane K, Yoshida Satoko, Shirasu Ken
RIKEN Center for Sustainable Resource Science, Yokohama, Japan.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Bio Protoc. 2017 May 5;7(9):e2260. doi: 10.21769/BioProtoc.2260.
is a facultative root parasitic plant in the Orobanchaceae family used as a model parasitic plant. Facultative root parasites form an invasive organ called haustorium on the lateral parts of their roots. To functionally characterize parasitic abilities, quantification of haustorium numbers is required. However, this task is quite laborious and time consuming. Here we describe an efficient protocol to induce haustorium by haustorium-inducing chemicals and host root exudate treatments in .
是列当科的一种兼性根寄生植物,用作模式寄生植物。兼性根寄生虫在其根的侧面形成一种称为吸器的侵入性器官。为了从功能上表征寄生能力,需要对吸器数量进行量化。然而,这项任务相当费力且耗时。在这里,我们描述了一种通过在……中用诱导吸器的化学物质和宿主根分泌物处理来诱导吸器的有效方案。