大波兰地区人乳头瘤病毒DNA的基因分型

Genotyping of human papillomavirus DNA in Wielkopolska region.

作者信息

Przybylski Marcin, Pruski Dominik, Millert-Kalinska Sonja, Madry Radoslaw, Lagiedo-Zelazowska Malgorzata, Sikora Jan, Zmaczynski Andrzej, Baran Rafal, Twardowska Hanna, Horbaczewska Anna, Jach Robert

机构信息

Department of Obstetrics and Gynecology, District Public Hospital in Poznan.

Department of Oncology, Poznan University of Medical Sciences, Poland.

出版信息

Ginekol Pol. 2021 Sep 20. doi: 10.5603/GP.a2021.0165.

DOI:10.5603/GP.a2021.0165

PMID:34541643

Abstract

OBJECTIVES

Human papillomavirus infection is one of the most common sexually transmitted diseases. Long-term exposure to the HPV leads to development of high-grade squamous intraepithelial lesions that can eventually transform into cervical cancer. The aim of the study was to assess the HPV genotype distribution in patients with abnormal pap smear and provide prospective study.

MATERIAL AND METHODS

We obtained material from 674 women who registered to Specialist Medical Practice in the years 2008-2020. The sample for the molecular test was collected using combi brush and forwarded to the independent, standardized laboratory. HPV detection was done using PCR followed by DNA enzyme immunoassay and reverse hybridization line probe assay for virus genotyping. Sequence analysis was performed to characterize virus genotypes in HPV - positive samples.

RESULTS

We found that 53% of patients tested positive for HPV. The percentage decreased with age. The following HPV types were the most common: HPV - 16 (24.5%), HPV - 53 (13.1%), HPV - 31 (10.3%), HPV - 51 (9.7%), HPV - 56 (9.5%). To our knowledge, this study is the largest assessment of HPV genotypes in Poland.

CONCLUSIONS

Our results suggest that type-specific, high-risk HPV DNA - based screening should focus on HPV types 16, 31, 51, 56.

摘要

目的

人乳头瘤病毒感染是最常见的性传播疾病之一。长期暴露于该病毒会导致高级别鳞状上皮内病变，最终可能转变为宫颈癌。本研究旨在评估巴氏涂片异常患者的人乳头瘤病毒基因型分布，并提供前瞻性研究。

材料与方法

我们收集了2008年至2020年在专科医疗诊所登记的674名女性的样本。使用组合刷收集用于分子检测的样本，并将其送至独立的标准化实验室。采用聚合酶链反应（PCR）检测人乳头瘤病毒，随后进行DNA酶免疫测定和反向杂交线性探针测定以进行病毒基因分型。对人乳头瘤病毒阳性样本进行序列分析以鉴定病毒基因型。

结果

我们发现53%的患者人乳头瘤病毒检测呈阳性。该百分比随年龄增长而降低。最常见的人乳头瘤病毒类型如下：人乳头瘤病毒16型（24.5%）、人乳头瘤病毒53型（13.1%）、人乳头瘤病毒31型（10.3%）、人乳头瘤病毒51型（9.7%）、人乳头瘤病毒56型（9.5%）。据我们所知，本研究是波兰对人乳头瘤病毒基因型进行的最大规模评估。