Departments of Pathology and Cell Biology, Emory University School of Medicine, Atlanta, GA, USA.
Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA, USA.
Methods Mol Biol. 2022;2364:149-158. doi: 10.1007/978-1-0716-1661-1_7.
The nematode Caenorhabditis elegans is one of the major model organisms in cell and developmental biology. This organism is easy to culture in laboratories and suitable for microscopic investigation of the cytoskeleton. Because the worms are small and transparent, the actin cytoskeleton in many tissues and cells can be observed with appropriate visualization techniques without sectioning or dissection. This chapter describes the introduction to representative methods for imaging the actin cytoskeleton in C. elegans and a protocol for staining worms with fluorescent phalloidin.
秀丽隐杆线虫是细胞和发育生物学的主要模式生物之一。这种生物在实验室中易于培养，并且适合于细胞骨架的显微镜研究。由于线虫很小且透明，因此可以使用适当的可视化技术观察许多组织和细胞中的肌动蛋白细胞骨架，而无需切片或解剖。本章介绍了在秀丽隐杆线虫中成像肌动蛋白细胞骨架的代表性方法，并提供了用荧光鬼笔环肽对线虫进行染色的方案。
