Department of Traditional Chinese Medicine, Daqing Oilfield General Hospital, 9 Zhongkang Street, Daqing 163001, China.
Day Clinic Centre, Daqing Oilfield General Hospital, 9 Zhongkang Street, Daqing 163001, China.
Pathog Dis. 2021 Sep 23;79(7). doi: 10.1093/femspd/ftab045.
Influenza A virus (H1N1), a swine-origin influenza A virus, causes seasonal epidemics that result in severe illnesses and deaths. Leonurine has been reported to function as an anti-inflammatory agent with protective effects on nervous, urinary and cardiovascular systems. However, the therapeutic effects of leonurine on the pneumonia caused by H1N1 infection remain unclear. Hematoxylin and eosin staining was performed to evaluate the lung injuries of mice infected by H1N1. The amount of immune cells was analyzed by flow cytometry. Enzyme-linked immunosorbent assay was used to evaluate the alteration of multiple cytokines in lung tissues. Real-time quantitative polymerase chain reaction assay was performed to investigate the ribonucleic acid (RNA) levels of certain genes. The protein levels in toll-like receptor 4/nuclear factor kappa-light-chain-enhancer of activated B cells (TLR4/NF-κB) signaling were estimated by western blot assay. Leonurine treatment significantly inhibited the mortality caused by H1N1 infection. Leonurine treatment (60 mg/kg) alleviated the lung injuries caused by virus infection. The inflammatory cell accumulation and cytokine expression were inhibited by the leonurine administration. Leonurine inhibited the mRNA expression of pro-inflammatory cytokines in the lung homogenates at day 5 postinfection. Leonurine regulated the TLR4/NF-κB signaling in the lung homogenates of H1N1-infected mice at day 5 postinfection. Leonurine protects against H1N1 infection-induced pneumonia in mice.
甲型 H1N1 流感病毒（简称 H1N1）是一种源于猪的流感病毒，可引起季节性流行，导致严重疾病和死亡。益母草碱已被报道具有抗炎作用，并对神经系统、泌尿系统和心血管系统具有保护作用。然而，益母草碱对 H1N1 感染引起的肺炎的治疗作用尚不清楚。通过苏木精-伊红染色评估 H1N1 感染小鼠的肺部损伤。通过流式细胞术分析免疫细胞数量。酶联免疫吸附试验用于评估肺组织中多种细胞因子的变化。实时定量聚合酶链反应检测特定基因的核糖核酸（RNA）水平。通过 Western blot 测定 Toll 样受体 4/核因子 kappa 轻链增强子的 B 细胞（TLR4/NF-κB）信号通路中的蛋白质水平。益母草碱治疗显著抑制了 H1N1 感染引起的死亡率。益母草碱（60mg/kg）治疗减轻了病毒感染引起的肺部损伤。益母草碱给药抑制了炎症细胞的聚集和细胞因子的表达。益母草碱在感染后第 5 天抑制了肺匀浆中促炎细胞因子的 mRNA 表达。益母草碱在感染后第 5 天调节了 H1N1 感染小鼠肺匀浆中的 TLR4/NF-κB 信号通路。益母草碱可预防 H1N1 感染诱导的小鼠肺炎。
