Zhang Xia, Zhang Li, Liu Shudi, Zhu Xiaozhen, Zhou Panpan, Cheng Xinyan, Zhang Renjie, Zhang Liangwei, Chen Lingxin
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.
J Hazard Mater. 2022 Feb 5;423(Pt B):127179. doi: 10.1016/j.jhazmat.2021.127179. Epub 2021 Sep 11.
Sulfur dioxide (SO) and its derivatives have long been considered as hazardous environmental pollutants but commonly used as food additives in safe dose range. They also could be produced from biological metabolism process of sulfur-containing amino acids. However, their physiological roles remain extremely obscure mainly due to lack of efficient tools for monitoring and imaging strategy establishment. Furthermore, most of current studies of this aspect focus on novel probe design or just imaging them rather than on the ins and outs. Therefore, there is a high significance of establishing highly sensitive detection strategy for monitoring SO derivatives in living systems, food and environment. Herein, we design a fluorescent probe MS-Bindol for sensitively detecting SO derivatives with a low detection limit (0.2 nM). We have established an imaging strategy for investigation of SO derivatives metabolism in living cells and zebrafish, providing visualize evidences and verified that SO derivatives could be synthetized from thiosulfate and glutathione(GSH) and be hardly consumed by using sulfite oxidase inhibitors (ferricyanide or arsenite). Moreover, the probe also exhibits excellent practicability in food as well as environmental samples. Our studies may help biologist for better understanding SO derivatives metabolism and deeply explore their physiological roles in biological systems.
二氧化硫（SO）及其衍生物长期以来一直被视为有害的环境污染物，但在安全剂量范围内通常用作食品添加剂。它们也可能由含硫氨基酸的生物代谢过程产生。然而，它们的生理作用仍然极其模糊，主要是由于缺乏用于监测和成像策略建立的有效工具。此外，目前这方面的大多数研究都集中在新型探针设计或仅仅对它们进行成像，而不是深入研究其来龙去脉。因此，建立高灵敏度检测策略以监测生物系统、食品和环境中的SO衍生物具有重要意义。在此，我们设计了一种荧光探针MS-Bindol，用于灵敏地检测SO衍生物，检测限低至0.2 nM。我们建立了一种成像策略，用于研究活细胞和斑马鱼中SO衍生物的代谢，提供了可视化证据，并证实SO衍生物可由硫代硫酸盐和谷胱甘肽（GSH）合成，并且使用亚硫酸盐氧化酶抑制剂（铁氰化物或亚砷酸盐）时几乎不被消耗。此外，该探针在食品以及环境样品中也表现出优异的实用性。我们的研究可能有助于生物学家更好地理解SO衍生物的代谢，并深入探索它们在生物系统中的生理作用。
