Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
J Hazard Mater. 2024 Dec 5;480:135975. doi: 10.1016/j.jhazmat.2024.135975. Epub 2024 Sep 26.
Sulfur dioxide (SO) has a wide range of applications in food additives and industrial production, and it is one of the main substances that form acid rain, causing serious harm to ecosystems and human health. Hence, it is necessary to construct an effective tool to quickly and accurately detect SO derivatives in environmental, food, and biological samples. In this study, fluorescent probe NPMQ was built to detect SO derivatives from nopinone with the merits of superior water solubility, high sensitivity (12 nM), excellent specificity, large Stokes shift (180 nm), and rapid response time (within 5 s). NPMQ was used to qualitatively and quantitatively detect SO derivatives in environmental water, soil and food samples. In addition, an electrospinning film was prepared with the probe NPMQ to image SO derivatives, and test strips are capable of rapidly, sensitively, and selectively detecting SO derivatives with the naked eye. Moreover, the probe NPMQ was used to visualize endogenous SO derivatives in Arabidopsis thaliana under Cd stress. Furthermore, the probe NPMQ was employed to image exogenous and endogenous SO derivatives in living Hela, HepG-2 cells, and zebrafish. This study develops an effective tool for monitoring SO derivatives in the environmental, food, and biological systems.
二氧化硫（SO）在食品添加剂和工业生产中有广泛的应用，它是形成酸雨的主要物质之一，对生态系统和人类健康造成严重危害。因此，有必要构建一种有效工具，以便快速准确地检测环境、食品和生物样品中的 SO 衍生物。在本研究中，构建了荧光探针 NPMQ，用于检测来自诺蒎酮的 SO 衍生物，其具有优异的水溶性、高灵敏度（12 nM）、出色的特异性、大斯托克斯位移（180nm）和快速的响应时间（<5s）。NPMQ 用于定性和定量检测环境水样、土壤和食品样品中的 SO 衍生物。此外，还使用探针 NPMQ 制备了电纺纤维膜，用于对 SO 衍生物进行成像，并且测试条能够通过肉眼快速、灵敏和选择性地检测 SO 衍生物。此外，该探针 NPMQ 用于可视化 Cd 胁迫下拟南芥中的内源性 SO 衍生物。进一步地，该探针 NPMQ 用于对活的 HeLa、HepG-2 细胞和斑马鱼中的外源性和内源性 SO 衍生物进行成像。本研究开发了一种用于监测环境、食品和生物系统中 SO 衍生物的有效工具。
