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从污染的地中海海洋沉积物中分离出的亚历山大深海线虫的宏基因组组装基因组 (MAG) 及其生物修复潜力。

Metagenome-assembled genome (MAG) of Oceancaulis alexandrii NP7 isolated from Mediterranean Sea polluted marine sediments and its bioremediation potential.

机构信息

Stazione Zoologica Anton Dohrn, Istituto Nationale di Biologia, Ecologia e Biotecnologie marine, 80121 Naples, Italy.

Department of Biotechnology, Institute for Microbial Biotechnology and Metagenomics (IMBM), University of the Western Cape, 7535 Bellville, Cape Town, South Africa.

出版信息

G3 (Bethesda). 2021 Sep 6;11(9). doi: 10.1093/g3journal/jkab210.

Abstract

Oceanicaulis alexandrii strain NP7 is a marine bacterium which belongs to the Hyphomonadaceae family and was isolated from sediments highly contaminated with metals and polycyclic aromatic hydrocarbons released for decades by industrial activities in the Gulf of Naples (Mediterranean Sea). Here, we report the partial genome sequence and annotation of O. alexandrii strain NP7 that contains a chromosome of 2,954,327 bp and encodes for 2914 predicted coding sequences (CDSs) and 44 RNA-encoding genes. Although the presence of some CDSs for genes involved in hydrocarbon degradation processes (e.g., alkB) have already been described in the literature associated with the Oceanicaulis, this is the first time that more than 100 genes involved in metal detoxification processes and hydrocarbon degradation are reported belonging to this genus. The presence of a heterogeneous set of genes involved in stress response, hydrocarbon degradation, heavy metal resistance, and detoxification suggests a possible role for O. alexandrii NP7 in the bioremediation of these highly contaminated marine sediments.

摘要

亚历山大海洋杆菌 NP7 菌株是一种海洋细菌,属于海单胞菌科,是从几十年前那不勒斯湾(地中海)工业活动释放的重金属和多环芳烃严重污染的沉积物中分离出来的。在这里,我们报告了 O. alexandrii 菌株 NP7 的部分基因组序列和注释,该基因组包含一条 2,954,327bp 的染色体,编码 2914 个预测编码序列(CDS)和 44 个 RNA 编码基因。尽管文献中已经描述了一些与海洋杆菌有关的参与烃类降解过程的基因(如 alkB)的 CDSs 的存在,但这是第一次报道超过 100 个参与金属解毒过程和烃类降解的基因属于这个属。参与应激反应、烃类降解、重金属抗性和解毒的基因的存在表明 O. alexandrii NP7 可能在这些高度污染的海洋沉积物的生物修复中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51f0/8496225/9c89773612f7/jkab210f1.jpg

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