Research Department, New England Biolabs, Ipswich, MA 01938-2723, USA.
CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), University Paris-Saclay, Gif-sur-Yvette 91198, France.
G3 (Bethesda). 2021 Sep 6;11(9). doi: 10.1093/g3journal/jkab226.
The Salmonella research community has used strains and bacteriophages over decades, exchanging useful new isolates among laboratories for the study of cell surface antigens, metabolic pathways and restriction-modification (RM) studies. Here we present the sequences of two laboratory Salmonella strains (STK005, an isolate of LB5000; and its descendant ER3625). In the ancestry of LB5000, segments of ∼15 and ∼42 kb were introduced from Salmonella enterica sv Abony 803 into S. enterica sv Typhimurium LT2, forming strain SD14; this strain is thus a hybrid of S. enterica isolates. Strains in the SD14 lineage were used to define flagellar antigens from the 1950s to the 1970s, and to define three RM systems from the 1960s to the 1980s. LB5000 was also used as a host in phage typing systems used by epidemiologists. In the age of cheaper and easier sequencing, this resource will provide access to the sequence that underlies the extensive literature.
沙门氏菌研究界数十年来一直使用菌株和噬菌体，在实验室之间交流有用的新分离株，用于研究细胞表面抗原、代谢途径和限制修饰（RM）研究。在这里，我们呈现了两个实验室沙门氏菌菌株（STK005，LB5000 的分离株；及其后代 ER3625）的序列。在 LB5000 的起源中，来自沙门氏菌 sv Abony 803 的约 15 和 42kb 片段被引入沙门氏菌 sv Typhimurium LT2 中，形成了 SD14 菌株；因此，该菌株是沙门氏菌分离株的杂种。在 20 世纪 50 年代至 70 年代，SD14 谱系中的菌株被用于定义鞭毛抗原，在 20 世纪 60 年代至 80 年代，还被用于定义三个 RM 系统。LB5000 也曾被用作噬菌体分型系统的宿主，被流行病学家使用。在测序成本更低、更容易的时代，这个资源将提供基础广泛的文献所依据的序列。
