State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, PR China.
J Biomed Nanotechnol. 2021 Aug 1;17(8):1668-1678. doi: 10.1166/jbn.2021.3128.
As infection induced by the implant will lead to operation failure, the implant material must be endowed with certain antibacterial properties. Hydroxyapatite (HA) mesoporous microspheres have been widely used in bone repair due to their advantages, including simple synthesis, good osteogenic properties and drug loading capacity. In this study, vancomycin hydrochloride-loaded mesoporous hydroxyapatite microspheres with micro/nanosurface structures were synthesized to increase osteogenic differentiation and antibacterial ability. Phytic acid (IP6) was used as a template to prepare mesoporous hydroxyapatite microspheres composed of fibres, flakes and smooth surfaces by the hydrothermal homogeneous precipitation method, and the corresponding specific surface areas were 65.20 m²/g, 75.13 m²/g and 71.27 m²/g, respectively. Vancomycin hydrochloride (Van) was used as the drug model to study the drug loading and release characteristics of the microspheres, as well as the antibacterial properties after treatment. In addition, during cocultivation with MC3T3-E1 preosteoblasts, HA microspheres assembled via flakes exhibited better cell compatibility, which promoted cell proliferation, alkaline phosphatase (ALP) activity, and the formation of calcium nodules and increased the expression of osteogenic differentiation-related proteins such as Runx-2, osteopontin (OPN) and collagen I (COL I). These results indicated that the HA microspheres prepared in this experiment have broad application prospects in drug delivery systems and bone repair.
由于植入物引起的感染会导致手术失败，因此植入物材料必须具有一定的抗菌性能。由于具有合成简单、成骨性能好、载药能力强等优点，羟基磷灰石（HA）介孔微球已广泛应用于骨修复领域。在这项研究中，通过水热均相沉淀法，以植酸（IP6）为模板，制备了具有微/纳米表面结构的载盐酸万古霉素介孔羟基磷灰石微球，其相应的比表面积分别为 65.20 m²/g、75.13 m²/g 和 71.27 m²/g。以盐酸万古霉素（Van）为药物模型，研究了微球的载药和释放特性以及处理后的抗菌性能。此外，在与 MC3T3-E1 前成骨细胞共培养过程中，片状组装的 HA 微球表现出更好的细胞相容性，促进了细胞增殖、碱性磷酸酶（ALP）活性、钙结节的形成，并增加了成骨分化相关蛋白如 Runx-2、骨桥蛋白（OPN）和胶原 I（COL I）的表达。这些结果表明，本实验制备的 HA 微球在药物传递系统和骨修复中有广阔的应用前景。
