Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, Institute of Insect Science and Technology and School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, China.
Proc Natl Acad Sci U S A. 2021 Sep 28;118(39). doi: 10.1073/pnas.2104461118.
It is well documented that the juvenile hormone (JH) can function as a gonadotropic hormone that stimulates vitellogenesis by activating the production and uptake of vitellogenin in insects. Here, we describe a phenotype associated with mutations in the JH receptor genes, and : the accumulation of mature eggs with reduced egg length in the ovary. JH signaling is mainly activated in ovarian muscle cells and induces gene expression in these cells. Meanwhile, JH signaling induces gene expression in the adult fat body, from which collagen IV is secreted and deposited onto the ovarian muscles. Laminin locally and collagen IV remotely contribute to the assembly of ovarian muscle extracellular matrix (ECM); moreover, the ECM components are indispensable for ovarian muscle contraction. Furthermore, ovarian muscle contraction externally generates a mechanical force to promote ovulation and maintain egg shape. This work reveals an important mechanism for JH-regulated insect reproduction.
已有大量文献证明，保幼激素（JH）可作为一种促性腺激素，通过激活昆虫卵黄蛋白原的产生和摄取来刺激卵黄发生。在这里，我们描述了与 JH 受体基因和突变相关的表型：卵巢中成熟卵的积累伴随着卵长的减少。JH 信号主要在卵巢肌肉细胞中被激活，并诱导这些细胞中基因的表达。同时，JH 信号诱导成年脂肪体中基因的表达，胶原蛋白 IV 从脂肪体中分泌并沉积到卵巢肌肉上。层粘连蛋白局部和胶原蛋白 IV 远程有助于卵巢肌肉细胞外基质（ECM）的组装；此外，ECM 成分对于卵巢肌肉收缩是必不可少的。此外，卵巢肌肉收缩产生向外的机械力以促进排卵和维持卵的形状。这项工作揭示了 JH 调节昆虫生殖的一个重要机制。
