Department of Otolaryngology, The First Affiliated Hospital of Soochow University, Soochow 215000, Jiangsu Province, China.
Aging (Albany NY). 2021 Sep 20;13(18):22176-22187. doi: 10.18632/aging.203521.
The present work was conducted to screen the potential biomarkers affecting nasopharyngeal carcinoma (NPC) progression through RNA-sequencing (RNA-seq), bioinformatic analysis and functional experiments.
Six normal samples and five NPC clinical samples were collected for RNA-seq analysis. The expression levels in both groups were determined through student's -test. We identified genes of < 0.01 as the differentially expressed genes (DEGs). In addition, gene set enrichment analysis (GSEA) was conducted. Afterwards, STRING V10 database was employed to extract protein interactions among the DEGs. Later, we established a protein-protein interaction (PPI) network, and used the Cytoscape software for network visualization. qRT-PCR was conducted to verify hub genes from clinical samples. Then, the function of CXCL10 in cell proliferation, apoptosis, invasion and migration was evaluated.
A total of 2024 DEGs were identified, among which, 1449 were down-regulated and 575 were up-regulated. The PPI was constructed, and the hub genes including Insulin Like Growth Factor 1 (), C-X-C Motif Chemokine Ligand 10 (), Interleukin 13 (), Intercellular Adhesion Molecule 1 (), G Protein Subunit Gamma Transducin 1 (), Matrix Metallopeptidase 1 (), Neurexin 1 () and Matrix Metallopeptidase 3 () were obtained. The expression levels and were significantly up-regulated in tumor tissues. High expression levels of and predicted poor prognosis of NPC patients. silencing suppressed NPC cell proliferation and migration.
may serve as a potential key gene affecting NPC genesis and progression.
本研究通过 RNA 测序（RNA-seq）、生物信息学分析和功能实验，筛选影响鼻咽癌（NPC）进展的潜在生物标志物。
收集 6 例正常样本和 5 例 NPC 临床样本进行 RNA-seq 分析。两组的表达水平通过学生 t 检验确定。我们将 < 0.01 的基因定义为差异表达基因（DEGs）。此外，还进行了基因集富集分析（GSEA）。然后，使用 STRING V10 数据库提取 DEGs 之间的蛋白质相互作用。之后，我们构建了一个蛋白质-蛋白质相互作用（PPI）网络，并使用 Cytoscape 软件进行网络可视化。通过 qRT-PCR 从临床样本中验证了关键基因。然后，评估了 CXCL10 在细胞增殖、凋亡、侵袭和迁移中的功能。
共鉴定出 2024 个 DEGs，其中 1449 个下调，575 个上调。构建了 PPI 网络，获得了包括胰岛素样生长因子 1（）、C-X-C 基序趋化因子配体 10（）、白细胞介素 13（）、细胞间黏附分子 1（）、G 蛋白亚基γ转导蛋白 1（）、基质金属蛋白酶 1（）、神经连接蛋白 1（）和基质金属蛋白酶 3（）在内的关键基因。在肿瘤组织中，和的表达水平显著上调。和的高表达水平预示着 NPC 患者的预后不良。沉默可抑制 NPC 细胞的增殖和迁移。
可能是影响 NPC 发生和进展的潜在关键基因。
