Sarfaraz Dorna, Karimian Mohammad, Farmohammadi Amir, Yaghini Jaber
Department of Periodontology, Torabinejad Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
J Oral Biosci. 2021 Dec;63(4):378-387. doi: 10.1016/j.job.2021.09.005. Epub 2021 Sep 20.
Chronic periodontitis (CP) is a common inflammatory disorder with a considerable impact of genetic variations in the interleukin family on predisposition to this disease. This study aimed to investigate the association between the -592C>A polymorphism of the interleukin 10 (IL-10) gene with CP risk in an Iranian population. This experimental study was followed by a meta-analysis and in silico examination.
In a case-control study, 270 subjects, including 135 patients with CP and 135 healthy controls, were enrolled. The -592C>A genotyping was performed using the PCR-RFLP method. In the meta-analysis, valid databases were systematically searched to identify relevant studies. Pooled odds ratios (ORs) with 95% confidence intervals (CIs) were computed to examine the association between -592C>A and CP. In silico analysis was conducted using bioinformatics tools to evaluate the impact of the -592C>A polymorphism on IL-10 gene function.
Our case-control study revealed a significant association between polymorphism and CP risk. Overall, we found significant associations between -592C>A genetic variation and CP and stratified meta-analysis. In silico analysis revealed that this polymorphism could change the pattern of the transcription binding site upstream of the IL-10 gene. It may also alter the hsa-miR-101-3p miRNA-targeted sequence upstream of IL-10.
Based on our results, the -592C>A variation in IL-10 may be a genetic risk factor for susceptibility to chronic periodontitis. However, further studies in different ethnicities and results adjusted for clinical and demographic characteristics are needed to obtain more accurate deductions.
慢性牙周炎（CP）是一种常见的炎症性疾病，白细胞介素家族的基因变异对该疾病的易感性有相当大的影响。本研究旨在调查伊朗人群中白细胞介素10（IL-10）基因-592C>A多态性与CP风险之间的关联。本实验研究之后进行了荟萃分析和计算机模拟分析。
在一项病例对照研究中，纳入了270名受试者，包括135名CP患者和135名健康对照。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行-592C>A基因分型。在荟萃分析中，系统检索有效数据库以识别相关研究。计算合并比值比（OR）及95%置信区间（CI），以检验-592C>A与CP之间的关联。使用生物信息学工具进行计算机模拟分析，以评估-592C>A多态性对IL-10基因功能的影响。
我们的病例对照研究揭示了多态性与CP风险之间存在显著关联。总体而言，我们发现-592C>A基因变异与CP之间存在显著关联，并进行了分层荟萃分析。计算机模拟分析表明，这种多态性可能会改变IL-10基因上游转录结合位点的模式。它还可能改变IL-10上游的hsa-miR-101-3p miRNA靶向序列。
根据我们的结果，IL-10基因的-592C>A变异可能是慢性牙周炎易感性的遗传危险因素。然而，需要在不同种族中进行进一步研究，并对临床和人口统计学特征进行调整后的结果，以获得更准确的推断。
