感染的基因组和表型特征
Genomic and Phenotypic Characteristics for Infections.
作者信息
Zhang Jiajie, Huang Yicheng, Xu Hao, Ying Shuaibing, Pan Hongying, Yu Wei
机构信息
Department of Infectious Diseases, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, People's Republic of China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
出版信息
Infect Drug Resist. 2021 Sep 14;14:3721-3726. doi: 10.2147/IDR.S331468. eCollection 2021.
BACKGROUND
(VV) is a causative agent of foodborne diseases with high mortality. The aim of this study was to investigate the genomic and phenotypic profiles of VV.
METHODS
Six VV isolates were collected and conducted whole-genome sequencing. Biofilm formation and anti-complement killing test were performed to evaluate the pathogenicity. Subsequently, 157 publicly available genomes of VV isolates were selected to determine the evolutionary relationship.
RESULTS
The resistant genes and were identified in six isolates. A total of 156 virulence genes were identified. However, there is no obvious difference between strains isolated from blood and puncture fluid. The tendency of growth for six isolates decreased with the lapse of time, while the biofilm formation increased. The genes and related to Flp pili were found in isolate 25506 and 30896, resulting in more obvious biofilm formation. In addition, the survival rate of 19656 was less than 20% due to lack of one genomic island including virulence genes () relevant to type VI secretion system (T6SS). Multi-locus sequence typing (MLST) revealed 95 different STs and 19 novel STs, indicating that the tendency of 163 isolates was sporadic. Further comparative genomics analysis clearly classified 163 isolates into three distinct evolutionary lineages.
CONCLUSION
VV infections were sporadic in humans and the environment. Virulence genes and related to T6SS were associated with pathogenicity phenotype of VV.
背景
（VV）是食源性疾病的病原体，死亡率很高。本研究的目的是调查VV的基因组和表型特征。
方法
收集了6株VV分离株并进行全基因组测序。进行生物膜形成和抗补体杀伤试验以评估致病性。随后，选择157个公开可用的VV分离株基因组来确定进化关系。
结果
在6株分离株中鉴定出抗性基因和。共鉴定出156个毒力基因。然而，从血液和穿刺液中分离出的菌株之间没有明显差异。6株分离株的生长趋势随时间推移而下降，而生物膜形成增加。在分离株25506和30896中发现了与Flp菌毛相关的基因和，导致生物膜形成更明显。此外，19656的存活率低于20%，因为缺少一个包括与VI型分泌系统（T6SS）相关的毒力基因（）的基因组岛。多位点序列分型（MLST）显示有95个不同的STs和19个新的STs，表明163株分离株的趋势是散发性的。进一步的比较基因组学分析清楚地将163株分离株分为三个不同的进化谱系。
结论
VV感染在人类和环境中是散发性的。与T6SS相关的毒力基因和与VV的致病性表型有关。
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