Department of Biophysics, Faculty of Medicine, Haliç University, Istanbul, 34445, Turkey.
Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, 22030, Turkey.
J Cancer Res Clin Oncol. 2021 Dec;147(12):3627-3637. doi: 10.1007/s00432-021-03808-y. Epub 2021 Sep 22.
The purpose of our study is to investigate the roles of IL-18 gene variations in bladder cancer development in Thrace population of Turkey.
This study was carried out with 103 bladder cancer patients and 81 healthy controls. Genotype distributions of IL-18 (-137 G/C) and IL-18 (-607 C/A) gene variations were determined using polymerase chain reaction (PCR) method.
The CC homozygous genotype for IL-18 (-607 C/A) gene variation was significantly higher in patients with bladder cancer compared to healthy controls (OR 0.345, 95% Cl 0.186-0.639, p = 0.001). Besides this, allele frequencies of IL-18 (-137 G/C) and IL-18 (-607 C/A) gene variations in patient with bladder cancer and healthy control groups were significantly different from the Hardy-Weinberg distribution (p < 0.05). For IL-18 (-137 G/C) and IL-18 (-607 C/A) gene variations, significant difference was determined between the bladder cancer patient and healthy control groups in terms of GC-CA (OR 0.381, 95% Cl 0.203-0.714, p = 0.002), GC-CC (OR 2.147, 95% Cl 1.013-4.550, p = 0.043), GG-AA (OR 0.431, 95% Cl 0.365-0.509, p = 0.049), and GG-CC (OR 2.476, 95% Cl 1.177-5.208, p = 0.015) haplotypes.
In our study, CC genotype of IL-18 (-607 C/A) gene variation was determined as genetic risk factor for bladder cancer development. In bladder cancer patient and healthy control groups, G and C allele frequencies of IL-18 (-137 G/C) gene variation, and C and A allele frequencies of IL-18 (-607 C/A) gene variation were determined significantly different from the Hardy-Weinberg distribution.
本研究旨在探讨白细胞介素-18（IL-18）基因变异在土耳其色雷斯人群膀胱癌发生中的作用。
本研究共纳入 103 例膀胱癌患者和 81 例健康对照者。采用聚合酶链反应（PCR）方法检测 IL-18（-137 G/C）和 IL-18（-607 C/A）基因变异的基因型分布。
与健康对照组相比，膀胱癌患者中 IL-18（-607 C/A）基因变异的 CC 纯合基因型显著升高（OR 0.345，95%CI 0.186-0.639，p=0.001）。此外，膀胱癌患者和健康对照组中 IL-18（-137 G/C）和 IL-18（-607 C/A）基因变异的等位基因频率与 Hardy-Weinberg 分布显著不同（p<0.05）。对于 IL-18（-137 G/C）和 IL-18（-607 C/A）基因变异，膀胱癌患者和健康对照组在 GC-CA（OR 0.381，95%CI 0.203-0.714，p=0.002）、GC-CC（OR 2.147，95%CI 1.013-4.550，p=0.043）、GG-AA（OR 0.431，95%CI 0.365-0.509，p=0.049）和 GG-CC（OR 2.476，95%CI 1.177-5.208，p=0.015）单倍型方面存在显著差异。
在本研究中，IL-18（-607 C/A）基因变异的 CC 基因型被确定为膀胱癌发生的遗传危险因素。在膀胱癌患者和健康对照组中，IL-18（-137 G/C）基因变异的 G 和 C 等位基因频率以及 IL-18（-607 C/A）基因变异的 C 和 A 等位基因频率与 Hardy-Weinberg 分布存在显著差异。
