Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture, Jilin Academy of Agricultural Sciences, Changchun, 130033, Jilin, People's Republic of China.
College of Plant Protection, Jilin Agricultural University, Changchun, 130118, People's Republic of China.
Arch Virol. 2021 Dec;166(12):3443-3447. doi: 10.1007/s00705-021-05215-7. Epub 2021 Sep 22.
Beauveria bassiana, an entomopathogenic fungus, is used for arthropod pest control worldwide. Here, we report the discovery and characterization of a novel double-stranded RNA (dsRNA) mycovirus, Beauveria bassiana chrysovirus 2 (BbCV-2), isolated from a Chinese B. bassiana strain. The genome sequence of the virus was determined by metagenomic sequencing, RT-PCR, and RACE cloning and was found to consist of four dsRNA segments that are 3441 bp, 2779 bp, 2925 bp, and 2688 bp long, respectively. Each dsRNA segment contains a single ORF. The ORF of dsRNA1 encodes a 1114-amino-acid (aa) protein (123.4 kDa) with a conserved RNA-dependent RNA polymerase (RdRp) motif, the sequence of which showed the highest identity of only 16.13% to that of Beauveria bassiana chrysovirus-1 (BbCV-1). The ORF of dsRNA2 encodes an 805-aa coat protein (CP) (84.7 kDa). The ORFs of dsRNAs 3 and 4 encodes proteins of undetermined function. The virus is a new member of the family Chrysoviridae from B. bassiana.
球孢白僵菌，一种昆虫病原真菌，在世界范围内被用于防治节肢动物害虫。在这里，我们报告了一种新型双链 RNA（dsRNA）真菌病毒的发现和特征，即球孢白僵菌 chrysovirus 2（BbCV-2），该病毒是从中国的一株球孢白僵菌中分离得到的。通过宏基因组测序、RT-PCR 和 RACE 克隆确定了病毒的基因组序列，由 4 个 dsRNA 片段组成，分别长 3441bp、2779bp、2925bp 和 2688bp。每个 dsRNA 片段都包含一个单一的 ORF。dsRNA1 的 ORF 编码一个 1114 个氨基酸（aa）的蛋白（123.4kDa），具有保守的 RNA 依赖性 RNA 聚合酶（RdRp）基序，其序列与 Beauveria bassiana chrysovirus-1（BbCV-1）的同源性仅为 16.13%。dsRNA2 的 ORF 编码一个 805 个氨基酸的外壳蛋白（CP）（84.7kDa）。dsRNA3 和 4 的 ORFs 编码功能未知的蛋白。该病毒是一种来自球孢白僵菌的 Chrysoviridae 科的新成员。
