Etemadi Ardavan, Imani Negar, Seyed Jafari Ehsan, Chiniforush Nasim
Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Photochem Photobiol. 2022 Mar;98(2):498-503. doi: 10.1111/php.13524. Epub 2021 Oct 23.
This in vitro study sought to assess the effect of repetitive PBMT on the viability of fibroblasts following aPDT with indocyanine green (ICG). In this in vitro experimental study, human gingival fibroblasts (HGFs) were obtained and incubated in a culture medium. After reaching 10 000 cells cm , the cells were divided into five groups of control, aPDT with ICG and 808 nm (energy density of 24 J cm ), PBMT immediately after aPDT, PBMT with 660 nm (energy density of 7.2 J cm ) immediately and 24 h after aPDT and PBMT immediately and 24 and 48 h after aPDT in 48-well plates. Cell viability was evaluated using the methyl thiazolyl tetrazolium (MTT) assay after 1, 4 and 7 days of incubation. Statistical analyses were performed using one-way ANOVA. Cell viability significantly decreased in group 2 (P < 0.002). We observed no significant increase in cell viability at any time point in group 3 (P > 0.05). Cell viability significantly increased in groups 4 and 5 after the first day of incubation (P < 0.000). Emission of 660 nm as PBMT for two and three times along with passage of time would increase the viability of HGFs following aPDT with ICG.
本体外研究旨在评估重复进行光生物调节疗法（PBMT）对使用吲哚菁绿（ICG）进行光动力疗法（aPDT）后成纤维细胞活力的影响。在这项体外实验研究中，获取人牙龈成纤维细胞（HGFs）并在培养基中培养。当细胞达到10000个/cm²后，将其分为五组：对照组、使用ICG和808nm（能量密度为24J/cm²）进行aPDT组、aPDT后立即进行PBMT组、aPDT后立即及24小时后使用660nm（能量密度为7.2J/cm²）进行PBMT组以及aPDT后立即、24小时和48小时使用660nm进行PBMT组，每组均培养于48孔板中。孵育1、4和7天后，使用噻唑蓝（MTT）法评估细胞活力。采用单因素方差分析进行统计分析。第2组细胞活力显著下降（P<0.002）。第3组在任何时间点细胞活力均未显著增加（P>0.05）。孵育第一天后，第4组和第5组细胞活力显著增加（P<0.000）。随着时间推移，使用660nm进行两次和三次PBMT可提高ICG介导的aPDT后HGFs的活力。
