Etemadi Ardavan, Koochak Hosseini Seyed Khashayar, Neshandar Morteza, Chiniforush Nasim
Department of Periodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
J Lasers Med Sci. 2024 Aug 31;15:e42. doi: 10.34172/jlms.2024.42. eCollection 2024.
This study aimed to assess the effect of repeated irradiations of 660 nm photobiomodulation therapy (PBMT) after photodynamic therapy (PDT) with curcumin on the viability of human gingival fibroblasts (HGFs). In this in vitro, experimental study, HGFs were cultured and assigned to five groups: One control group with no intervention and four experimental groups of PDT with curcumin, PBMT (660 nm laser irradiation) immediately after PDT, PBMT immediately and 24 hours after PDT and PBMT immediately and 24 hours and 48 hours after PDT. Cell viability was assessed after 1, 4, and 7 days using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by one-way ANOVA. On day 1, the control group had no significant difference with one-time (=1.00), two-time (=1.00), and three-time (=0.88) laser irradiation groups. On day 4, the difference between the control and one-time (<0.001), two-time (<0.001) and three-time (=0.02) laser irradiation groups was statistically significant, suggesting more cell viability in test groups. On day 7, the three-time laser irradiation group showed significant cell viability compared to the other two test groups but not with the control group (=0.98). PBMT with 660 nm laser irradiation after PDT with curcumin would increase the viability of HGFs by increasing the frequency of irradiation.
本研究旨在评估姜黄素光动力疗法（PDT）后重复进行660 nm光生物调节疗法（PBMT）对人牙龈成纤维细胞（HGFs）活力的影响。在这项体外实验研究中，培养HGFs并将其分为五组：一组为无干预的对照组，四组为姜黄素PDT实验组，分别是PDT后立即进行PBMT（660 nm激光照射）、PDT后立即及24小时后进行PBMT、PDT后立即及24小时和48小时后进行PBMT。使用甲基噻唑基四氮唑（MTT）法在1、4和7天后评估细胞活力。数据采用单因素方差分析。第1天，对照组与单次（=1.00）、两次（=1.00）和三次（=0.88）激光照射组无显著差异。第4天，对照组与单次（<0.001）、两次（<0.001）和三次（=0.02）激光照射组之间的差异具有统计学意义，表明测试组细胞活力更高。第7天，与其他两个测试组相比，三次激光照射组显示出显著的细胞活力，但与对照组（=0.98）相比无差异。姜黄素PDT后进行660 nm激光照射的PBMT可通过增加照射频率提高HGFs的活力。
