woodland 二倍体草莓（ Fragaria vesca L. ）中 SPATULA （ FvSPT ）和 SPIRAL （ FvSPR ）基因的启动子分析。
Promoter analysis of the SPATULA (FvSPT) and SPIRAL (FvSPR) genes in the woodland diploid strawberry (Fragaria vesca L.).
机构信息
Centre for Agricultural Genomics and Biotechnology, Faculty of Agricultural and Food Science and Environmental Management, University of Debrecen, P.O. Box 12, Nyíregyháza, 4400, Hungary.
Independent Researcher, Miki-cho post office, Ikenobe 3011-2, P. O. Box 7, Kagawa-ken, 761-0799, Japan.
出版信息
Biol Futur. 2021 Sep;72(3):373-384. doi: 10.1007/s42977-021-00089-x. Epub 2021 Jun 9.
The aim of this study was to identify transcription factor (TF) binding sites and cis-regulatory elements (CREs) on the promoters of FvSPR1-like2 (SPIRAL) and FvSPT (SPATULA) genes in the woodland diploid strawberry (Fragaria vesca L.). We identified: (1) MYB59, WRKY25 and WRKY8 TFs which play a role in ethylene signaling; (2) ARF family of TFs which play a role in ARF-mediated auxin signaling on the promoter of FvSPR1-like2 gene; (3) ARR family of TFs which play a role in cytokinin signaling; (4) ERF family of TFs which play a role in ethylene signaling on the promoter of FvSPT. This bioinformatic analysis of TFs and CREs may provide a better understanding of the function of genes involved in, and the mechanism underlying, non-climateric ripening during strawberry fruit maturation.
本研究旨在鉴定林生二倍体草莓（ Fragaria vesca L. ） FvSPR1-like2（SPIRAL）和 FvSPT（SPATULA）基因启动子上转录因子（TF）结合位点和顺式调控元件（CRE）。我们鉴定出：（1）在 FvSPR1-like2 基因启动子上，发挥乙烯信号作用的 MYB59、WRKY25 和 WRKY8 TFs；（2）ARF 家族 TFs，在 ARF 介导的生长素信号中发挥作用；（3）ARR 家族 TFs，在细胞分裂素信号中发挥作用；（4）在 FvSPT 基因启动子上，发挥乙烯信号作用的 ERF 家族 TFs。对 TF 和 CRE 的生物信息学分析，可能有助于深入了解草莓果实成熟过程中非跃变型成熟相关基因的功能及其机制。
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