Am J Vet Res. 2021 Oct;82(10):846-852. doi: 10.2460/ajvr.82.10.846.
To compare urine concentrations of fibrinogen (uFIB) and interleukin-6 (uIL-6) between dogs with risk factors for enterococcal bacteriuria and healthy dogs.
Banked urine samples with negative aerobic culture results from 8 dogs with urolithiasis, 9 dogs with anatomic abnormalities of the lower portion of the urinary tract (LUT), 10 dogs with LUT neoplasia, and 21 healthy control dogs.
Urine creatinine concentration (uCrea) was determined by an automated biochemical analyzer, and uFIB and uIL-6 were determined by dog-specific ELISAs. The uFIB:uCrea and uIL-6:uCrea ratios were calculated for each sample to normalize intersample differences in urine concentration and were compared among the 4 experimental groups.
Median uFIB:uCrea ratios for dogs with urolithiasis (0.72; interquartile [25th to 75 percentile] range [IQR], 0.46 to 3.48) and LUT neoplasia (6.16; IQR, 3.89 to 12.75), but not for dogs with LUT anatomic abnormalities (0.48; IQR, 0.27 to 0.69), were significantly greater than that for control dogs (0.17; IQR, 0.07 to 0.39). Median uIL-6: uCrea ratios for dogs with urolithiasis (0.48; IQR, 0.18 to 1.61), LUT anatomic abnormalities (0.25; IQR, 0.17 to 0.33), and LUT neoplasia (0.25; IQR, 0.12 to 1.01) were significantly greater than that for control dogs (0.08; IQR, 0.06 to 0.11).
The uFIB and uIL-6 in dogs with risk factors for enterococcal bacteriuria were generally greater than corresponding values in control dogs. Further investigation is necessary to determine the role of fibrinogen in enterococcal colonization of the urinary tract of dogs.
比较有肠球菌性菌尿风险因素的犬与健康犬的尿纤维蛋白原（uFIB）和白细胞介素-6（uIL-6）浓度。
来自 8 只患有尿路结石、9 只存在下尿路（LUT）解剖异常、10 只患有 LUT 肿瘤和 21 只健康对照犬的尿液样本，这些样本均为银行储存的、有氧培养结果为阴性的尿液样本。
通过自动化生化分析仪测定尿肌酐浓度（uCrea），并通过犬特异性 ELISA 测定 uFIB 和 uIL-6。计算每个样本的 uFIB:uCrea 和 uIL-6:uCrea 比值，以标准化尿液浓度的样本间差异，并比较 4 个实验组之间的差异。
患有尿路结石（0.72；四分位距 [25%至 75%]范围 [IQR]，0.46 至 3.48）和 LUT 肿瘤犬（6.16；IQR，3.89 至 12.75）的 uFIB:uCrea 比值中位数显著大于健康对照组犬（0.17；IQR，0.07 至 0.39），但患有 LUT 解剖异常犬（0.48；IQR，0.27 至 0.69）的 uFIB:uCrea 比值中位数无显著差异。患有尿路结石（0.48；IQR，0.18 至 1.61）、LUT 解剖异常（0.25；IQR，0.17 至 0.33）和 LUT 肿瘤犬（0.25；IQR，0.12 至 1.01）的 uIL-6:uCrea 比值中位数显著大于健康对照组犬（0.08；IQR，0.06 至 0.11）。
有肠球菌性菌尿风险因素的犬的 uFIB 和 uIL-6 通常高于健康对照组犬。需要进一步研究以确定纤维蛋白原在犬肠球菌尿路定植中的作用。
