Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.
Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China; Institute of Biological Resources, Jiangxi Academy of Science, Nanchang 330096, China.
Plant Physiol Biochem. 2021 Oct;167:946-954. doi: 10.1016/j.plaphy.2021.09.021. Epub 2021 Sep 20.
B-box (BBX) genes play important roles in plant growth, light morphogenesis, and environmental stress responses. Ginkgo (Ginkgo biloba L.) is known as a living fossil species that has a strong ability to adapt to environmental changes and tolerate harsh conditions. In this study, we chose this species to investigate the function of the GbBBX25 gene. We isolated the BBX gene from ginkgo and named it GbBBX25; this gene consists of an 819 bp open reading frame (ORF) that encodes 273 amino acids with two B-box domains but no CCT domain. GbBBX25 was localized in only the nucleus. The expression of GbBBX25 transcripts was observed in the leaves and was significantly enhanced under salt stress conditions. To further verify its function, we overexpressed the GbBBX25 gene in Populus davidiana × Populus bolleana and found that the transgenic Populus had greater soluble sugar levels and higher peroxidase (POD) activity in response to salt stress than nontransgenic (NT) Populus. Five genes related to salt stress were induced in transgenic plants with significantly higher expression levels than those in NT plants. This finding suggests that GbBBX25 improves the salt adaptation abilities of transgenic Populus and provides a scientific basis for related research.
B-box (BBX) 基因在植物生长、光形态发生和环境胁迫响应中发挥重要作用。银杏（Ginkgo biloba L.）被称为活化石物种，具有很强的适应环境变化和耐受恶劣条件的能力。在这项研究中，我们选择了这个物种来研究 GbBBX25 基因的功能。我们从银杏中分离出 BBX 基因，并将其命名为 GbBBX25；该基因由 819bp 的开放阅读框（ORF）组成，编码 273 个氨基酸，具有两个 B-box 结构域但没有 CCT 结构域。GbBBX25 仅定位于细胞核中。GbBBX25 转录本的表达在叶片中观察到，并在盐胁迫条件下显著增强。为了进一步验证其功能，我们在胡杨（Populus davidiana × Populus bolleana）中过表达了 GbBBX25 基因，发现转基因胡杨在应对盐胁迫时具有更高的可溶性糖水平和过氧化物酶（POD）活性，而非转基因（NT）胡杨则没有。与盐胁迫相关的五个基因在转基因植物中被诱导，其表达水平明显高于 NT 植物。这一发现表明，GbBBX25 提高了转基因胡杨的耐盐能力，为相关研究提供了科学依据。
