Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China.
Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China; Research Center for Pomology, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Qian Hu Hou Cun No. 1, Nanjing, 210014, China.
Plant Sci. 2021 Dec;313:111092. doi: 10.1016/j.plantsci.2021.111092. Epub 2021 Oct 12.
WD40 transcription factors are an ancient protein family whose members play important roles in plant growth and stress resistance. In this study, a new WD40 gene was cloned from Ginkgo biloba L. via the rapid amplification of cDNA ends (RACE) technique. This gene was 824 bp in length and encoded 109 amino acids. Sequence alignment and phylogenetic analysis showed that this transcription factor was most similar to the LWD1 protein, and it was thus named GbLWD1-like. This gene was expressed mainly in the leaves, followed by the roots. Phenotypic analysis showed that the transgenic plants grew better, were taller, and had significantly more roots than the control check (CK) plants. Moreover, the transgenic plants were more tolerant to salt stress than the CK plants. After 11 days of salt treatment, all the leaves of the CK plants had dried up and fallen off, whereas in the transgenic lines, only the edges of the bottom leaves had turned yellow. Under salt stress, the expression levels of some genes related to salt tolerance were higher in the transgenic plants than in the CK plants. This study suggests that the GbLWD1-like gene may be related to the growth potential and improved salt tolerance of plants and may play an important role in the response to adversity.
WD40 转录因子是一个古老的蛋白质家族，其成员在植物生长和抗逆性方面发挥着重要作用。本研究通过快速扩增 cDNA 末端（RACE）技术从银杏中克隆出一个新的 WD40 基因。该基因长 824bp，编码 109 个氨基酸。序列比对和系统进化分析表明，该转录因子与 LWD1 蛋白最为相似，因此将其命名为 GbLWD1-like。该基因主要在叶片中表达，其次是根部。表型分析表明，与对照（CK）植株相比，转基因植株生长更好，株高更高，根更多。此外，转基因植株对盐胁迫的耐受性强于 CK 植株。盐处理 11 天后，CK 植株的所有叶片都干枯脱落，而在转基因系中，只有下部叶片的边缘变黄。在盐胁迫下，转基因植株中一些与耐盐性相关的基因的表达水平高于 CK 植株。本研究表明，GbLWD1-like 基因可能与植物的生长潜力和耐盐性提高有关，并可能在逆境响应中发挥重要作用。
