Eriksdotter-Nilsson M, Jonsson G, Dahl D, Björklund H
Department of Histology, Karolinska Institutet, Stockholm, Sweden.
Int J Dev Neurosci. 1986;4(4):353-62. doi: 10.1016/0736-5748(86)90052-3.
Treatment of pregnant rats on gestation day 15 with methylazoxymethanol (MAM) leads to a marked microencephaly in the offspring with a considerable atrophy in cerebral cortex, hippocampus and striatum. The development of the astrocytic populations in these atrophic regions was studied by means of immunohistochemistry using an antiserum against glial fibrillary acidic protein (GFA). The distribution and density of GFA-positive structures were not notably altered in the parietal cortex, hippocampal formation and striatum after prenatal MAM-treatment as compared to control. Also the individual astrocytes were morphologically similar in experimental and control animals in all regions analyzed. We suggest that an adjustment of the astrocytic development has occurred in response to the changed neuronal environment. Alternatively, MAM-treatment may affect neuronal and glial precursor cells leading to a seemingly normal astrocytic cell density.
在妊娠第15天用甲基氧化偶氮甲醇（MAM）处理怀孕大鼠，会导致后代出现明显的小头畸形，大脑皮层、海马体和纹状体出现相当程度的萎缩。通过使用抗胶质纤维酸性蛋白（GFA）抗血清的免疫组织化学方法，研究了这些萎缩区域星形胶质细胞群体的发育情况。与对照组相比，产前MAM处理后，顶叶皮层、海马结构和纹状体中GFA阳性结构的分布和密度没有明显改变。在所有分析区域中，实验动物和对照动物的单个星形胶质细胞在形态上也相似。我们认为，星形胶质细胞的发育已经发生了调整，以应对变化的神经元环境。或者，MAM处理可能会影响神经元和神经胶质前体细胞，导致星形胶质细胞密度看似正常。
