Shahbazi Parisa, Nematollahi Ahmad, Arshadi Sanaz, Farhang Hosein Hashemzadeh, Shahbazfar Amir Ali
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University Tabriz branch, Tabriz, Iran.
Iran J Parasitol. 2021 Apr-Jun;16(2):279-288. doi: 10.18502/ijpa.v16i2.6318.
A specific treatment has not yet developed for cryptosporidiosis, and some of the used drugs had side effects in immunodeficient patients. The goal of an appropriate remedy is to remove symptoms and improve immune responses in hosts. The current study was designed to evaluate the therapeutic efficacy of ethanolic extract in experimentally infected immunosuppressed mice.
Thirty six NMRI mice, 4-6 wk old, were randomly divided into six equal groups. C1: uninfected, treated control; C2: infected, untreated control; T1, T2, T3, and P: infected, treated with 0.2, 2, and 20 mg/ml extract, and 5mg/ml paromomycin, respectively. Mice were experimentally infected by oral administration of 10 oocysts/animal of and treated orally for eight days per 12h, starting 12h before experimental infection. The presence of oocyst shedding, weight gain/loss, and the histopathology of ileum sections were examined.
Results revealed that oocyst shedding was significantly (<0.05) reduced in treatment groups. There was no significant difference between the mean of weight gain/loss in the infected control and treated groups. Histopathological analysis of ileum sections further supported the parasitological findings.
had acceptable efficacy as a therapeutic agent for cryptosporidiosis.
隐孢子虫病尚未开发出特异性治疗方法，一些已使用的药物在免疫缺陷患者中存在副作用。合适治疗方法的目标是消除症状并改善宿主的免疫反应。本研究旨在评估乙醇提取物对实验性感染免疫抑制小鼠的治疗效果。
将36只4 - 6周龄的NMRI小鼠随机分为六组。C1：未感染，治疗对照组；C2：感染，未治疗对照组；T1、T2、T3和P组：感染，分别用0.2、2和2mg/ml提取物以及5mg/ml巴龙霉素治疗。通过每只动物口服10个卵囊对小鼠进行实验性感染，并在实验感染前12小时开始，每12小时口服治疗8天。检查卵囊排出情况、体重增加/减轻以及回肠切片的组织病理学。
结果显示，治疗组的卵囊排出显著减少（<0.05）。感染对照组和治疗组的体重增加/减轻平均值之间无显著差异。回肠切片的组织病理学分析进一步支持了寄生虫学研究结果。
作为隐孢子虫病的治疗剂具有可接受的疗效。
