Shen Rui, Lin Dehui, Liu Zhe, Zhai Honglei, Yang Xingbin
Shaanxi Engineering Laboratory for Food Green Processing and Safety Control, Shaanxi Key Laboratory for Hazard Factors Assessment in Processing and Storage of Agricultural Products, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, China.
Department of Pediatrics, Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX, United States.
Front Nutr. 2021 Sep 7;8:734620. doi: 10.3389/fnut.2021.734620. eCollection 2021.
In this study, the anti-solvent precipitation and a simple complex method were applied for the preparation of bacterial cellulose nanofiber/soy protein isolate (BCNs/SPI) colloidal particles. Fourier transform IR (FT-IR) showed that hydrogen bonds generated in BCNs/SPI colloidal particles the anti-solvent precipitation were stronger than those generated in BCNs/SPI colloidal particles self-assembled by a simple complex method. Meanwhile, the crystallinity, thermal stability, and contact angle of BCNs/SPI colloidal particles the anti-solvent precipitation show an improvement in comparison with those of BCNs/SPI colloidal particles a simple complex method. BCNs/SPI colloidal particles the anti-solvent precipitation showed enhanced gel viscoelasticity, which was confirmed by dynamic oscillatory measurements. Furthermore, high internal phase Pickering emulsions (HIPEs) were additionally stable due to their stabilization by BCNs/SPI colloidal particles the anti-solvent precipitation. Since then, HIPEs stabilized by BCNs/SPI colloidal particles the anti-solvent precipitation were used for the delivery of curcumin. The curcumin-loaded HIPEs showed a good encapsulation efficiency and high 2,2-diphenyl-1-picrylhydrazyl (DPPH) removal efficiency. Additionally, the bioaccessibility of curcumin was significantly increased to 30.54% after the encapsulation using the prepared HIPEs. Therefore, it can be concluded that the anti-solvent precipitation is an effective way to assemble the polysaccharide/protein complex particles for the stabilization of HIPEs, and the prepared stable HIPEs showed a potential application in the delivery of curcumin.
在本研究中，采用反溶剂沉淀法和一种简单的复合方法制备了细菌纤维素纳米纤维/大豆分离蛋白（BCNs/SPI）胶体颗粒。傅里叶变换红外光谱（FT-IR）表明，通过反溶剂沉淀法制备的BCNs/SPI胶体颗粒中产生的氢键比通过简单复合方法自组装的BCNs/SPI胶体颗粒中产生的氢键更强。同时，与通过简单复合方法制备的BCNs/SPI胶体颗粒相比，通过反溶剂沉淀法制备的BCNs/SPI胶体颗粒的结晶度、热稳定性和接触角都有所提高。通过动态振荡测量证实，通过反溶剂沉淀法制备的BCNs/SPI胶体颗粒表现出增强的凝胶粘弹性。此外，由于通过反溶剂沉淀法制备的BCNs/SPI胶体颗粒的稳定作用，高内相Pickering乳液（HIPEs）具有额外的稳定性。此后，通过反溶剂沉淀法制备的BCNs/SPI胶体颗粒稳定的HIPEs被用于姜黄素的递送。负载姜黄素的HIPEs表现出良好的包封效率和较高的2,2-二苯基-1-苦基肼（DPPH）清除效率。此外，使用制备的HIPEs进行包封后，姜黄素的生物可及性显著提高至30.54%。因此，可以得出结论，反溶剂沉淀法是组装多糖/蛋白质复合颗粒以稳定HIPEs的有效方法，并且制备的稳定HIPEs在姜黄素递送方面显示出潜在的应用前景。
