Richmond A, Lawson D H, Nixon D W, Stevens J S, Chawla R K
Cancer Res. 1983 May;43(5):2106-12.
Extracts of conditioned medium (CM) from Hs0294 human malignant melanoma cells stimulate [3H]thymidine incorporation and an increase in cell number in serum-depleted Hs0294 cells. This activity is acid and heat stable, nonproteolytic, protease sensitive, contains disulfide bonds and elutes broadly from a Bio-Gel P-30 column with an approximate molecular weight range of 6,000 to 25,000. Hs0294 CM also stimulates [3H]thymidine incorporation in nonmalignant human nevus cells and normal rat kidney fibroblast cells but not in human fibroblasts. There was only limited competition with 125I-epidermal growth factor in binding assays. Hs0294 CM extracts stimulate anchorage-independent growth in normal rat kidney fibroblast cells in soft agar but not in Hs0294 cells, nevus cells, or human fibroblasts. This second activity elutes from the Bio-Gel P-30 column in two positions with apparent molecular weights of 27,000 and 11,000.
来自Hs0294人恶性黑色素瘤细胞的条件培养基（CM）提取物可刺激血清饥饿的Hs0294细胞中[3H]胸苷掺入并增加细胞数量。该活性对酸和热稳定，无蛋白水解作用，但对蛋白酶敏感，含有二硫键，并且从Bio-Gel P-30柱上宽泛洗脱下来时，其近似分子量范围为6,000至25,000。Hs0294 CM还可刺激非恶性人痣细胞和正常大鼠肾成纤维细胞中[3H]胸苷掺入，但对人成纤维细胞无此作用。在结合试验中，与125I-表皮生长因子的竞争有限。Hs0294 CM提取物可刺激正常大鼠肾成纤维细胞在软琼脂中进行不依赖贴壁的生长，但对Hs0294细胞、痣细胞或人成纤维细胞无此作用。该第二种活性从Bio-Gel P-30柱上在两个位置洗脱下来，表观分子量分别为27,000和11,000。
