Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China.
Int J Syst Evol Microbiol. 2021 Sep;71(9). doi: 10.1099/ijsem.0.005026.
A Gram-positive, aerobic, heterotrophic, non-endospore-forming, rod-shaped and indole-acetic acid-producing strain, designated NEAU-184, was isolated from marine sand collected in Sanya, PR China, and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NEAU-184 should be assigned to the genus and formed a distinct branch with its closest neighbour, NBRC 106452 (99.1 %). 2,4-Diaminobutyric acid, d-alanine, d-glutamic acid and glycine were detected in cell-wall hydrolysate and glucose, rhamnose and xylose were detected in whole-cell hydrolysate. The polar lipids were found to contain diphosphatidylglycerol, glycolipid, phosphatidylglycerol and two unidentified lipids. The major menaquinone was MK-12 and the minor menaquinones were MK-13 and MK-11. The predominant fatty acids were anteiso-C, anteiso-C and iso-C. The DNA G+C content was 71.5 mol%. Furthermore, the strain could be clearly distinguished from its closely related type strains by the combination of DNA-DNA hybridization results and some phenotypic characteristics. Meanwhile, the strain has the ability to produce indole-acetic acid (0.334mg ml). Therefore, strain NEAU-184 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain NEAU-184 (=CGMCC 4.7505=JCM 32546) as the type strain.
一株革兰氏阳性、需氧、异养、不产芽孢、杆状、能产吲哚乙酸的菌株 NEAU-184，从中国三亚海洋沙样中分离得到，采用多相分类法对其分类地位进行了研究。基于 16S rRNA 基因序列数据分析的系统发育结果表明，菌株 NEAU-184 应归入 属，与最近的模式菌株 NBRC 106452（99.1%）形成了一个独特的分支。细胞壁水解产物中检测到 2,4-二氨基丁酸、D-丙氨酸、D-谷氨酸和甘氨酸，全细胞水解产物中检测到葡萄糖、鼠李糖和木糖。极性脂中含有双磷脂酰甘油、糖脂、磷脂酰甘油和两种未鉴定的脂类。主要的menaquinone 为 MK-12，次要的menaquinone 为 MK-13 和 MK-11。主要脂肪酸为 anteiso-C15:0、anteiso-C17:0 和 iso-C15:0。DNA G+C 含量为 71.5mol%。此外，该菌株可通过 DNA-DNA 杂交结果和一些表型特征与密切相关的模式菌株相区分。同时，该菌株具有产生吲哚乙酸（0.334mg ml）的能力。因此，菌株 NEAU-184 代表了 属的一个新种，命名为 sp. nov.，其模式菌株为 NEAU-184（=CGMCC 4.7505=JCM 32546）。
