Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2247-2253. doi: 10.1099/ijsem.0.003987. Epub 2020 Feb 25.
Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-A and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau. Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C. The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to , respectively. In the 16S rRNA gene and polygenetic trees, strains MF30-A and MF845 were clearly distinct from other species. The two strains could not produce acid from arbutin, d-fructose, D-sucrose, glycogen, salicin or starch. Production of β-glucosidase by these strains was negative. The major fatty acids of these strains were anteiso-C, anteiso-C and iso-C. Strain MF30-A contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones. The major polar lipids in strain MF30-A were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid. The G+C contents of the DNA of strains MF30-A and MF845 were 69.8 mol% and 69.7 mol%, respectively. The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus were far below the respective thresholds of 95 and 70 %, respectively. All genotypic and phenotypic data indicated that strains MF30-A and MF845 should be classified as novel members of the genus , for which the name sp. nov. is proposed. The type strain is MF30-A (=CGMCC 1.16469=DSM 106183).
两株需氧、革兰氏阳性、过氧化氢酶阳性、不运动的杆状细菌菌株，分别命名为 MF30-A 和 MF845，从青藏高原采集的高原兔肠道内容物中分离得到。这两株菌在需氧条件下、pH7.0 和 28°C 下生长最佳。这两株菌的 16S rRNA 基因序列与 的相似性最高，分别为 98.5%和 98.4%。在 16S rRNA 基因和多基因树中，菌株 MF30-A 和 MF845 与其他物种明显不同。这两株菌不能从熊果苷、D-果糖、D-蔗糖、糖原、水杨苷或淀粉中产生酸。这两株菌不产β-葡萄糖苷酶。这两株菌的主要脂肪酸为 anteiso-C、anteiso-C 和 iso-C。菌株 MF30-A 含有半乳糖、鼠李糖和核糖作为细胞壁糖，MK-12 和 MK-11 作为主要的menaquinones。菌株 MF30-A 的主要极性脂为双磷脂酰甘油、磷脂酰甘油和糖脂，而肽聚糖含有丙氨酸、谷氨酸、甘氨酸和 2,4-二氨基丁酸。菌株 MF30-A 和 MF845 的 DNA 的 G+C 含量分别为 69.8 mol%和 69.7 mol%。两株菌与所有可用的属基因组的平均核苷酸同一性和数字 DNA-DNA 相关性值均远低于各自的 95%和 70%的阈值。所有基因型和表型数据表明，菌株 MF30-A 和 MF845 应归类为属的新成员，建议将其命名为 sp. nov.。模式菌株为 MF30-A（=CGMCC 1.16469=DSM 106183）。
