Crawford P C, Clark W B
J Dent Res. 1986 Feb;65(2):105-8. doi: 10.1177/00220345860650020101.
The implantation of Actinomyces viscosus strain T14V-J1 was studied in mice in vivo. The optimum age range for implantation was from 40 to 80 days. The minimum infective dose of strain T14V-J1 required for colonization in all inoculated animals (ID100) was 5 X 10(8) CFU for 40-day-old mice and 1 X 10(9) CFU for 100-day-old mice. The high ID100 required for implantation of strain T14V-J1 in these mice is consistent with the finding that tooth surfaces of these animals are not colonized by detectable indigenous Actinomyces. The infection could also be transmitted to non-infected mice housed with infected mice. This in vivo model can be used to evaluate the potential for reducing or preventing Actinomyces colonization on teeth by vaccination with fimbrial adhesins.
在小鼠体内研究了粘性放线菌T14V-J1菌株的植入情况。植入的最佳年龄范围是40至80天。在所有接种动物中定殖所需的T14V-J1菌株的最小感染剂量（ID100），对于40日龄小鼠为5×10⁸CFU，对于100日龄小鼠为1×10⁹CFU。T14V-J1菌株在这些小鼠中植入所需的高ID100与这些动物的牙齿表面未被可检测到的本土放线菌定殖这一发现一致。感染也可传播给与感染小鼠同笼饲养的未感染小鼠。这种体内模型可用于评估通过接种菌毛粘附素来减少或预防牙齿上放线菌定殖的潜力。
