Wheeler T T, Clark W B, Birdsell D C
Infect Immun. 1979 Sep;25(3):1066-74. doi: 10.1128/iai.25.3.1066-1074.1979.
Adsorption of Actinomyces viscosus strains T14V and T14AV to hydroxyapatite (HA) surfaces was studied, using an adsorption model based on the Langmuir adsorption isotherm. Data generally followed the adsorption model as judged by high correlation coefficients obtained for both strains to most of the treated surfaces studied. The number of binding sites for strains T14V and T14AV cells to human saliva-treated HA was similar to that for untreated HA. The affinity of strain T14V for saliva-treated HA was tenfold greater than the affinity of strain T14AV for that surface. To approximate the pellicle of the gingival crevice and margin and to determine whether adherence by strain T14V was to specific saliva or serum receptors, experimental pellicles were formed on HA by saliva/serum mixtures. The number of binding sites on the saliva/serum-treated HA remained the same as for the saliva-treated surface. Although the affinity of strain T14V cells for the saliva/serum HA surface remained generally the same as the affinity for the HA treated with saliva alone, the affinity of strain T14AV cells decreased further as the serum content increased. Strain T14V cell numbers adsorbed to serum-treated HA, and albumin-treated HA were less than those adsorbed to saliva-treated HA, indicating that the adherence by strain T14V was to specific saliva receptors. In vivo results from streptomycin-resistant mutants of both strains T14V and T14AV confirmed in vitro results using saliva-serum pellicles. Pretreatment of strain T14V with proteolytic enzymes and heat inhibited adherence to saliva-treated HA, suggesting that the adherence receptor(s) on the cell surface of strain T14V is protein in nature.
采用基于朗缪尔吸附等温线的吸附模型，研究了黏性放线菌菌株T14V和T14AV对羟基磷灰石（HA）表面的吸附情况。通过两种菌株与大多数研究的处理表面获得的高相关系数判断，数据总体上符合吸附模型。菌株T14V和T14AV细胞与人唾液处理的HA的结合位点数与未处理的HA相似。菌株T14V对唾液处理的HA的亲和力比对该表面的菌株T14AV的亲和力大十倍。为了近似牙龈沟和边缘的薄膜，并确定菌株T14V的黏附是否针对特定的唾液或血清受体，通过唾液/血清混合物在HA上形成实验薄膜。唾液/血清处理的HA上的结合位点数与唾液处理的表面相同。尽管菌株T14V细胞对唾液/血清HA表面的亲和力总体上与对仅用唾液处理的HA的亲和力相同，但随着血清含量的增加，菌株T14AV细胞的亲和力进一步降低。吸附到血清处理的HA和白蛋白处理的HA上的菌株T14V细胞数量少于吸附到唾液处理的HA上的细胞数量，表明菌株T14V的黏附是针对特定的唾液受体。菌株T14V和T14AV的链霉素抗性突变体的体内结果证实了使用唾液 - 血清薄膜的体外结果。用蛋白水解酶和热对菌株T14V进行预处理可抑制其对唾液处理的HA的黏附，这表明菌株T14V细胞表面的黏附受体本质上是蛋白质。
